In vitro Assessment of RNA Polymerase I Activity
Author: Marzia Govoni,
date: 02/05/2017,
view: 5896,
Q&A: 0
[Abstract] In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs (mRNAs) and small RNAs, while RNA polymerase III transcribes the genes for transfer RNAs and for the ...
Acid Extraction of Total Histone from Colon Cancer HCT116 Cells
Authors: Lin-Lin Cao and
Wei-Guo Zhu,
date: 11/20/2016,
view: 8022,
Q&A: 0
[Abstract] Histone acid extraction assay is a popular method to determine histone modification levels in mammalian cells. It includes three steps: first, histones are released from chromatin by sulfuric acid; trichloroacetate (TCA) is then added to precipitate histones; and finally, histones are dissolved in double-distilled H2O (ddH2 ...
Analysis of Protein Stability by the Cycloheximide Chase Assay
Authors: Shih-Han Kao,
Wen-Lung Wang,
Chi-Yuan Chen,
Yih-Leong Chang,
Yi-Ying Wu,
Yi-Ting Wang,
Shu-Ping Wang,
Alexey I Nesvizhskii,
Yu-Ju Chen,
Tse-Ming Hong and
Pan-Chyr Yang,
date: 01/05/2015,
view: 53721,
Q&A: 1
[Abstract] Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much popularity in cancer research. Here we present a ...
Hypoxia Studies with Pimonidazole in vivo
Authors: Kristina Y. Aguilera and
Rolf A. Brekken,
date: 10/05/2014,
view: 21275,
Q&A: 0
[Abstract] Therapy-induced hypoxia drives changes in the tumor microenvironment that contribute to the poor response to therapy. Hypoxia is capable of driving the expression and/or activation of specific signaling cascades (e.g., c-Met, Axl, CTGF), the recruitment of tumor promoting immune cells, and the induction of cell survival pathways including ...