Microbiology

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    Protocols in Current Issue
    Analysis of Replicative Intermediates of Adeno-associated Virus through Hirt Extraction and Southern Blotting
    [Abstract] Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates can be detected. This detailed protocol ...
    Isolation of Genomic DNA from Mycobacterium Species
    Authors:  Pawan Kumar, Soumitra Marathe and Sangeeta Bhaskar, date: 03/05/2016, view: 11502, Q&A: 1
    [Abstract] Isolation of genomic DNA from Mycobacterium species has been a tedious procedure. This can primarily be attributed to thick and waxy cell wall of mycobacteria which hampers lysis of the bacterial cell. We have tested various approaches to isolate mycobacterial DNA and based on this, an optimized protocol is presented here. This protocol ...
    Quantification of Total and 2-LTR (Long terminal repeat) HIV DNA, HIV RNA and Herpesvirus DNA in PBMCs
    Authors:  Marta Massanella, Sara Gianella, Steven M. Lada, Douglas D. Richman and Matthew C. Strain, date: 06/05/2015, view: 9206, Q&A: 0
    [Abstract] Almost all individuals infected with human immunodeficiency virus (HIV) are also infected with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have included characterizing and measuring the latent HIV reservoir and understanding the association between asymptomatic replication of CMV (and other herpesvirus, including ...



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