Plant Science


    Protocols in Current Issue
    Virus-based MicroRNA Silencing
    Authors:  Jinping Zhao and Yule Liu, date: 01/20/2016, view: 7504, Q&A: 0
    [Abstract] Virus-based microRNA silencing (VbMS) is a viable and prompt method to screen and characterize the function of microRNAs (miRNAs) in plants. The Tobacco rattle virus (TRV)-based VbMS method was originally developed by the Yule Liu's group (Sha et al., 2014) using miRNA target mimic (TM) methodology. Here, we describe the ...
    In vitro Reconstitution Assay of miRNA Biogenesis by Arabidopsis DCL1
    Authors:  Tian Wang, Claudia Castillo-González, Lan You, Rui Li, Liwei Wen, Hongliang Zhu and Xiuren Zhang, date: 04/20/2015, view: 6851, Q&A: 0
    [Abstract] microRNAs (miRNAs) are small non-coding RNAs, regulating most if not all, biological processes in eukaryotic organisms. miRNAs are initially processed from primary transcripts (pri-miRNAs) to produce miRNA precursors (pre-miRNAs), that are further processed into miRNA and its complementary strands (miRNA/*). In Arabidopsis, and possibly other ...
    Extraction of Small RNA and qPCR Validation of miRNAs in Vigna mungo
    Authors:  Sujay Paul, Anirban Kundu and Amita Pal, date: 03/05/2015, view: 7985, Q&A: 0
    [Abstract] Small RNAs like microRNAs (miRNAs), small interfering RNAs (siRNAs) and other noncoding RNAs including snRNA and snoRNA have tremendous impact on eukaryotic gene regulation. Extraction of high quality small RNAs is an important prerequisite for experimental analyses of miRNAs. This will prevent RNA degradation and remove associated contaminations ...
    Virus-induced Gene Silencing (VIGS) in Phalaenopsis Orchids
    Authors:  Ming-Hsien Hsieh, Zhao-Jun Pan, Hsin-Hung Yeh and Hong-Hwa Chen, date: 12/20/2014, view: 9478, Q&A: 0
    [Abstract] This is a protocol to produce stable silencing efficacy and efficiency for VIGS using CymMV as a silencing vector for floral functional genomics in Phalaenopsis orchids. This protocol is established based on a method created by Lu et al. (2007), and then modified by Hsieh et al. (2013a; 2013b), Lu et al. (2012) ...
    Targeted Gene Mutation in Rice Using a CRISPR-Cas9 System
    Authors:  Kabin Xie, Bastian Minkenberg and Yinong Yang, date: 09/05/2014, view: 25668, Q&A: 0
    [Abstract] RNA-guided genome editing (RGE) using bacterial type II cluster regularly interspaced short palindromic repeats (CRISPR)–associated nuclease (Cas) has emerged as a simple and versatile tool for genome editing in many organisms including plant and crop species. In RGE based on the Streptococcus pyogenes CRISPR-Cas9 system, the Cas9 ...
    A High Resolution Short Interfering RNA (siRNA) Detection Method from Virus-infected Plants
    Authors:  Vinay Panwar and Guus Bakkeren, date: 10/20/2013, view: 10082, Q&A: 0
    [Abstract] Plant viruses are strong inducers as well as targets of RNA silencing. In plants RNA silencing acts as a natural defense mechanism against viral infection and is associated with accumulation of virus-specific small interfering RNAs (siRNAs). The continuing discoveries, increasing awareness and interest in the regulatory roles of non-coding small ...

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