Plant Science

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    Protocols in Current Issue
    Telomere-mediated Chromosomal Truncation via Agrobacterium tumefaciens or Particle Bombardment to Produce Engineered Minichromosomes in Plants
    [Abstract] Minichromosomes are small, autonomously functioning chromosomes that exist separately from the normal chromosomal set. Creation of minichromosomes in plants relies on telomere truncation to remove the chromosome arms and the native telomere sequence and replace them with a transgene together with a new telomere sequence to generate a modifiable ...
    Rice Meiotic Chromosome Spread Preparation of Pollen Mother Cells
    Authors:  Xingwang Li and Changyin Wu, date: 07/20/2014, view: 7845, Q&A: 0
    [Abstract] In this protocol, we describe a simple and efficient method for meiotic chromosome spread preparation in rice pollen mother cells. Meiotic chromosome preparation by spreading itself is an important technique for plant cytogenetics (Higgins et al., 2004; Chelysheva et al., 2012; Wang et al., 2009); furthermore, it is a ...
    Micrococcal Nuclease (MNase) Assay of Arabidopsis thaliana Nuclei
    Authors:  Laia Armengot and Jordi Moreno-Romero, date: 04/05/2013, view: 17899, Q&A: 2
    [Abstract] Micrococcal nuclease (MNase) is able to produce double-strand breaks within nucleosome linker regions. The efficiency of MNase digestion depends on the degree of chromatin compaction, being more easily digested the regions of less compacted chromatin. The MNase protocol described here can be used to asses changes in the chromatin structure of ...



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