Developmental Biology

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    Protocols in Current Issue
    Determining Oxidative Damage by Lipid Peroxidation Assay in Rat Serum
    Authors:  Qian Tang, Yu-Wen Su and Cory J. Xian, date: 06/20/2019, view: 608, Q&A: 0
    [Abstract] It has been well-established that malondialdehyde (MDA), which is generated during the process of lipid peroxidation, is a commonly known biomarker for oxidative stress. Therefore, the serum levels of MDA are detected by using the lipid peroxidation assay with commercially available kit to determine the induction of oxidative stress in rat models.
    Quantification of the Humidity Effect on HR by Ion Leakage Assay
    Authors:  Musoki Mwimba and Xinnian Dong, date: 04/05/2019, view: 826, Q&A: 0
    [Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
    Measurement of Intracellular ROS in Caenorhabditis elegans Using 2’,7’-Dichlorodihydrofluorescein Diacetate
    Authors:  Dong Suk Yoon, Myon-Hee Lee and Dong Seok Cha, date: 03/20/2018, view: 4827, Q&A: 1
    [Abstract] Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum of diseases such as cancer, ...
    Oxidative Stress Assays (arsenite and tBHP) in Caenorhabditis elegans
    Authors:  Collin Yvès Ewald, John M. Hourihan and T. Keith Blackwell, date: 07/05/2017, view: 6001, Q&A: 0
    [Abstract] Cells and organisms face constant exposure to reactive oxygen species (ROS), either from the environment or as a by-product from internal metabolic processes. To prevent cellular damage from ROS, cells have evolved detoxification mechanisms. The activation of these detoxification mechanisms and their downstream responses represent an overlapping ...
    P-body and Stress Granule Quantification in Caenorhabditis elegans
    Authors:  Matthias Rieckher and Nektarios Tavernarakis, date: 01/20/2017, view: 6822, Q&A: 0
    [Abstract] Eukaryotic cells contain various types of cytoplasmic, non-membrane bound ribonucleoprotein (RNP) granules that consist of non-translating mRNAs and a versatile set of associated proteins. One prominent type of RNP granules is Processing bodies (P bodies), which majorly harbors translationally inactive mRNAs and an array of proteins mediating mRNA ...



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