Murine Pancreatic Islets Transplantation under the Kidney Capsule
[Abstract] Type 1 diabetes (T1D) is an autoimmune disease caused by the lack of insulin-producing pancreatic beta cells leading to systemic hyperglycemia. Pancreatic islet transplantation is a valid therapeutic approach to restore insulin loss and to promote adequate glycemic control. Pancreatic islet transplantation in mice is an optimal preclinical model ...
Tumorigenicity Assay in Nude Mice
Authors: Feng Du,
Xiaodi Zhao and
Daiming Fan,
date: 07/05/2017,
view: 16294,
Q&A: 0
[Abstract] Tumorigenicity refers to the ability of cultured cells to develop viable tumors in immune-deficient animals. The goal of this protocol is to illustrate tumorigenicity assay by subcutaneous tumor-cell-transplantation in nude mice. Target cells are transplanted to 6-week-old nude mice subcutaneously and the tumor growth is monitored over a period of ...
Intracaecal Orthotopic Colorectal Cancer Xenograft Mouse Model
Authors: Hsin-Wei Liao and
Mien-Chie Hung,
date: 06/05/2017,
view: 10019,
Q&A: 1
[Abstract] The host microenvironment plays a prominent role in tumor growth, angiogenesis, invasion, metastasis, and response to therapy. Orthotopic tumor model mimics the natural environment of tumor development and provides an effective approach to investigate tumor pathophysiology and develop therapeutic strategies. This protocol describes the technique ...
Whole Mammary Gland Transplantation in Mice Protocol
[Abstract] Whole Mammary Gland Transplantation involves transplanting an excised mammary gland into another, more suitable host. This method can be used to extend the life of a mammary gland past the mouse’s life span by transplanting the mammary gland of an older mouse into a young healthy mouse. As you can see in the video below (Video 1), by attaching it ...
A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis
[Abstract] Prostate cancer is one of the most common cancers in men in the United States. Comprehensive understanding of the biology contributing to prostate cancer will have important clinical implications. Animal models have greatly impacted our knowledge of disease and will continue to be a valuable resource for future studies. Herein, we describe a ...