Cell Biology

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    Protocols in Current Issue
    Murine Hair Follicle Derived Stem Cell Transplantation onto the Cornea Using a Fibrin Carrier
    [Abstract] The goal of this protocol is to establish a procedure for cultivating stem cells on a fibrin carrier to allow for eventual transplantation to the eye. The ability to transfer stem cells to a patient is critical for treatment for a variety of disorders and wound repair. We took hair follicle stem cells from the vibrissae of transgenic mice ...
    Murine Pancreatic Islets Transplantation under the Kidney Capsule
    [Abstract] Type 1 diabetes (T1D) is an autoimmune disease caused by the lack of insulin-producing pancreatic beta cells leading to systemic hyperglycemia. Pancreatic islet transplantation is a valid therapeutic approach to restore insulin loss and to promote adequate glycemic control. Pancreatic islet transplantation in mice is an optimal preclinical model ...
    Tumorigenicity Assay in Nude Mice
    Authors:  Feng Du, Xiaodi Zhao and Daiming Fan, date: 07/05/2017, view: 11513, Q&A: 0
    [Abstract] Tumorigenicity refers to the ability of cultured cells to develop viable tumors in immune-deficient animals. The goal of this protocol is to illustrate tumorigenicity assay by subcutaneous tumor-cell-transplantation in nude mice. Target cells are transplanted to 6-week-old nude mice subcutaneously and the tumor growth is monitored over a period of ...
    Intracaecal Orthotopic Colorectal Cancer Xenograft Mouse Model
    Authors:  Hsin-Wei Liao and Mien-Chie Hung, date: 06/05/2017, view: 6870, Q&A: 1
    [Abstract] The host microenvironment plays a prominent role in tumor growth, angiogenesis, invasion, metastasis, and response to therapy. Orthotopic tumor model mimics the natural environment of tumor development and provides an effective approach to investigate tumor pathophysiology and develop therapeutic strategies. This protocol describes the technique ...
    Whole Mammary Gland Transplantation in Mice Protocol
    Authors:  Hayley Hansford, Yan Hong, Frances Kittrell, Daniel Medina and Fariba Behbod, date: 06/05/2017, view: 4559, Q&A: 0
    [Abstract] Whole Mammary Gland Transplantation involves transplanting an excised mammary gland into another, more suitable host. This method can be used to extend the life of a mammary gland past the mouse’s life span by transplanting the mammary gland of an older mouse into a young healthy mouse. As you can see in the video below (Video 1), by attaching it ...
    A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis
    Authors:  Robert M. Hughes, Brian W. Simons and Paula J. Hurley, date: 02/20/2017, view: 6124, Q&A: 0
    [Abstract] Prostate cancer is one of the most common cancers in men in the United States. Comprehensive understanding of the biology contributing to prostate cancer will have important clinical implications. Animal models have greatly impacted our knowledge of disease and will continue to be a valuable resource for future studies. Herein, we describe a ...
    Allogeneic Transplantation of Testicular Hyperplasia in rag1 Mutant Zebrafish
    Authors:  Toshihiro Kawasaki and Noriyoshi Sakai, date: 11/05/2016, view: 4590, Q&A: 0
    [Abstract] Allogeneic organ transplantation is a powerful tool for clinical and basic research studies. However, the graft is often rejected by the host organism. Here, we describe a protocol that uses immunodeficient rag1 mutant zebrafish. These zebrafish escaped rejection, which made it possible to successfully transplant fragments of an ...



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