Biochemistry

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    Protocols in Current Issue
    RNA Cap Methyltransferase Activity Assay
    Authors:  Jackson B. Trotman and Daniel R. Schoenberg, date: 03/20/2018, view: 2488, Q&A: 0
    [Abstract] Methyltransferases that methylate the guanine-N7 position of the mRNA 5’ cap structure are ubiquitous among eukaryotes and commonly encoded by viruses. Here we provide a detailed protocol for the biochemical analysis of RNA cap methyltransferase activity of biological samples. This assay involves incubation of cap-methyltransferase-containing ...
    In vitro Assays for the Detection of Calreticulin Exposure, ATP and HMGB1 Release upon Cell Death
    Authors:  Yuting Ma and Heng Yang, date: 12/20/2016, view: 10358, Q&A: 1
    [Abstract] Accumulating evidence is revealing the essential role of immune system in cancer treatment. Certain chemotherapeutic drugs can potently induce the release of ‘cell death associated molecular patterns’ (CDAMPs), which accompanies cancer cell demise. CDAMPs can engage corresponding receptors on immune cells and stimulate immune responses to achieve ...
    Intracellular Assessment of ATP Levels in Caenorhabditis elegans
    Authors:  Konstantinos Palikaras and Nektarios Tavernarakis, date: 12/05/2016, view: 5947, Q&A: 0
    [Abstract] Eukaryotic cells heavily depend on adenosine triphosphate (ATP) generated by oxidative phosphorylation (OXPHOS) within mitochondria. ATP is the major energy currency molecule, which fuels cell to carry out numerous processes, including growth, differentiation, transportation and cell death among others (Khakh and Burnstock, 2009). Therefore, ATP ...
    Determination of Intracellular ATP Levels in Mycelium of Fusarium oxysporum
    Authors:  Carmen Ruiz-Roldan and M. Isabel G. Roncero, date: 07/20/2016, view: 5290, Q&A: 0
    [Abstract] Glycolysis provides metabolites for energy production via oxidative phosphorylation during vegetative growth of Fusarium oxysporum. Therefore, determination of intracellular ATP levels might be of valuable help to analyze regulation of glycolysis/gluconeogenesis pathways. The protocol described here can be applied to other filamentous ...



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