Immunology

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    Protocols in Current Issue
    Isolation of PBMCs Using Vacutainer® Cellular Preparation Tubes (CPTTM)
    Authors:  Alaina Puleo, Chantia Carroll, Holden Maecker and Rohit Gupta, date: 01/20/2017, view: 28482, Q&A: 0
    [Abstract] Peripheral blood mononuclear cell (PBMC) isolation is commonly done via density gradient centrifugation over Ficoll-Hypaque, a labor-intensive procedure that requires skilled technicians and can contribute to sample variability. Cellular Preparation Tubes (CPTs) are Vacutainer blood draw tubes that contain Ficoll-Hypaque and a gel plug that ...
    Isolation of Joint-infiltrating Cells
    Authors:  Aoi Akitsu and Yoichiro Iwakura, date: 09/05/2016, view: 7961, Q&A: 0
    [Abstract] Infiltration of leukocytes into joints is one of the main features of autoimmune inflammatory arthritis. Here, we describe the protocol for isolation of joint-infiltrating cells in mice. This protocol is useful to analyze cell surface antigens and intracellular cytokines by flow cytometry.
    Preparation of Single Cell Suspensions from Mouse Aorta
    Authors:  Desheng Hu, Changjun Yin, Sarajo Mohanta, Christian Weber and Andreas J. R. Habenicht, date: 06/05/2016, view: 12499, Q&A: 0
    [Abstract] Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by lipid deposition, plaque formation, and immune cell infiltration. Innate and adaptive immune cells infiltrate the artery during development of the disease. Moreover, advanced disease leads to formation of artery tertiary lymphoid organs in the adventitia ...



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