Immunology

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    Protocols in Current Issue
    Vaginal HSV-2 Infection and Tissue Analysis
    Authors:  Marie Beck Iversen, Søren Riis Paludan and Christian Kanstrup Holm, date: 07/05/2017, view: 4247, Q&A: 0
    [Abstract] The vaginal murine HSV-2 infection model is very useful in studying mucosal immunity against HSV-2 (Overall et al., 1975; Renis et al., 1976; Parr and Parr, 2003). Histologically, the vagina of Depo-Provera-treated mice is lined by a single layer of mucus secreting columnar epithelial cells overlying two to three layers of ...
    Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts
    Authors:  Joseph C. Chen and Nadia R. Roan, date: 10/20/2015, view: 10769, Q&A: 0
    [Abstract] Purification and culture of endometrial epithelial cells (eEC) and stromal fibroblasts (eSF) from endometrial biopsies allows for downstream cell-specific in vitro studies. The utility of this protocol is the ease with which cells are purified without contamination from unwanted cell types, and the ability to use patient-paired eEC and ...



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