Cancer Biology

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    Protocols in Current Issue
    A CRISPR Competition Assay to Identify Cancer Genetic Dependencies
    Authors:  Vishruth Girish and Jason M. Sheltzer, date: 07/20/2020, view: 4258, Q&A: 0
    [Abstract] The CRISPR/Cas9 system is a powerful tool for genome editing, wherein the RNA-guided nuclease Cas9 can be directed to introduce double-stranded breaks (DSBs) at a targeted locus. In mammalian cells, these DSBs are typically repaired through error-prone processes, resulting in insertions or deletions (indels) at the targeted locus. Researchers can ...



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