Cancer Biology

Categories

    Protocols in Current Issue
    Calvarial Bone Implantation and in vivo Imaging of Tumor Cells in Mice
    Authors:  Kyoko Hashimoto, Shingo Sato, Hiroki Ochi, Shu Takeda and Mitsuru Futakuchi, date: 02/05/2019, view: 952, Q&A: 0
    [Abstract] Bone is one of common metastasis sites for many types of cancer. In bone metastatic microenvironment, tumor-bone interactions play a significant role in the regulation of osteolytic or osteoblastic bone metastasis. In order to investigate the direct interaction between tumor cells and bone tissue, it is essential to generate appropriate animal ...
    3D Stroma Invasion Assay
    Authors:  Yvette May Coulson-Thomas and Vivien Jane Coulson-Thomas, date: 03/20/2017, view: 4185, Q&A: 0
    [Abstract] We have developed a 3D co-culture system composed of fibroblasts and colorectal cancer cells that enables us to study the desmoplastic reaction. This method also enables us to study the influence of the desmoplastic reaction on the migration of colorectal cancer cells through the surrounding stroma. This protocol has been previously published ...
    Analysis of Cancer Stromal Reaction Using an O-ring Co-culture Assay
    Author:  Vivien Jane Coulson-Thomas, date: 02/20/2017, view: 4434, Q&A: 0
    [Abstract] We have developed a 2D heterotypic co-culture technique between fibroblasts and cancer cells that enables the study of the stromal reaction. For such, stromal cells are seeded and cultured immediately around a tumour cell line, and the cells establish cell-cell contacts, as well as a gradient of soluble factors throughout the stromal cells, ...
    3D Gel Invasion Assay of Gastric Cancer Cells with Fibroblasts
    Author:  Masamitsu Tanaka, date: 05/05/2016, view: 6962, Q&A: 1
    [Abstract] Cancer tissue is composed of cancer cells and a large number of stromal cells including fibroblasts. In order to understand the relationship between fibroblasts and cancer cells during invasion of the stroma, 3D gel invasion assay is useful. Most tumors are associated with a biologically active type of fibroblasts known as cancer-associated ...
    In vivo Lineage-tracing Studies in a Cancer Stem Cell Population in Neuroblastoma
    Authors:  Danielle M. Hsu, Jason M. Shohet and Eugene S. Kim, date: 04/20/2014, view: 6323, Q&A: 0
    [Abstract] Tumors are comprised of heterogeneous subpopulations that may exhibit differing capacity for differentiation, self-renewal, and tumorigenicity. In vivo lineage-tracing studies are a powerful tool for defining the role of tumor subpopulations in tumor growth and as targets for therapeutic agents. This protocol describes using a ...
    Isolation and Immortalization of Fibroblasts from Different Tumoral Stages
    Authors:  Fernando Calvo, Steven Hooper and Erik Sahai, date: 04/05/2014, view: 11763, Q&A: 0
    [Abstract] Tumour microenvironment and cancer-associated fibroblasts in particular exhibit tumour promoting abilities that are not present in their normal counterparts (Calvo et al., 2013; Hanahan and Coussens, 2012). Therefore, functional and molecular characterization of the modifications occurring in fibroblasts during tumour progression is ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.