Cancer Biology

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    Protocols in Current Issue
    Ca2+ Measurements in Mammalian Cells with Aequorin-based Probes
    Authors:  Anna Tosatto, Rosario Rizzuto and Cristina Mammucari, date: 03/05/2017, view: 5975, Q&A: 0
    [Abstract] Aequorin is a Ca2+ sensitive photoprotein suitable to measure intracellular Ca2+ transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, thus allowing an accurate measurement of Ca2+ uptake and release of different ...
    Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime
    Authors:  Thomas S. Blacker, Tunde Berecz, Michael R. Duchen and Gyorgy Szabadkai, date: 01/20/2017, view: 9894, Q&A: 0
    [Abstract] NADH and NADPH are redox cofactors, primarily involved in catabolic and anabolic metabolic processes respectively. In addition, NADPH plays an important role in cellular antioxidant defence. In live cells and tissues, the intensity of their spectrally-identical autofluorescence, termed NAD(P)H, can be used to probe the mitochondrial redox state, ...
    In vivo Efficacy Studies in Cell Line and Patient-derived Xenograft Mouse Models
    Authors:  Elizabeth A. Tovar, Curt J. Essenburg and Carrie Graveel, date: 01/05/2017, view: 11372, Q&A: 0
    [Abstract] In vivo xenograft models derived from human cancer cells have been a gold standard for evaluating the genetic drivers of cancer and are valuable preclinical models for evaluating the efficacy of cancer therapeutics. Recently, patient-derived tumorgrafts from multiple tumor types have been developed and shown to more accurately ...



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