In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells
[Abstract] Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based ...
In vivo Leukemogenesis Model Using Retrovirus Transduction
Authors: Hiroshi Okuda and
Akihiko Yokoyama,
date: 12/05/2017,
view: 5031,
Q&A: 0
[Abstract] Various genetic alterations such as chromosomal translocation cause leukemia. For examples, gene rearrangements of the mixed-lineage leukemia (MLL) gene generate MLL fusion genes, whose products are potent oncogenic drivers in acute leukemia. To better understand the mechanism of disease onset, several murine leukemia models ...
Xenograft Mouse Model of Human Uveal Melanoma
Authors: Yao Chen,
Xiao Liu,
Ling Gao and
Yongqing Liu,
date: 11/05/2017,
view: 4682,
Q&A: 0
[Abstract] Uveal melanoma (UM) is a malignant intraocular tumor in adults. Metastasis develops in almost half of the patients and over 90% of the metastases are in the liver. With the advances in molecular targeting therapy for melanoma, a proper metastasis animal model is of increasing importance for testing the accuracy and effectiveness of systemic ...
Glioma Induction by Intracerebral Retrovirus Injection
Authors: Ravinder K Verma ,
Fanghui Lu and
Qing Richard Lu,
date: 07/20/2017,
view: 5863,
Q&A: 0
[Abstract] Glioblastoma (GBM) is the most common primary brain cancer in adults and has a poor prognosis. It is characterized by a high degree of cellular infiltration that leads to tumor recurrence, atypical hyperplasia, necrosis, and angiogenesis. Despite aggressive treatment modalities, current therapies are ineffective for GBM. Mouse GBM models not only ...
A Novel Mouse Skin Graft Model of Vascular Tumors Driven by Akt1
Authors: Thuy L. Phung and
Sriram Ayyaswamy,
date: 07/05/2017,
view: 6829,
Q&A: 0
[Abstract] To investigate whether endothelial Akt1 activation is sufficient to induce vascular tumor formation in the skin, we have developed a skin graft model in which a skin fragment from transgenic donor mice with inducible and endothelial cell-specific overexpression of activated Akt1 (myrAkt1) is grafted into the skin of wild type recipient mice. The ...
Intracaecal Orthotopic Colorectal Cancer Xenograft Mouse Model
Authors: Hsin-Wei Liao and
Mien-Chie Hung,
date: 06/05/2017,
view: 8116,
Q&A: 1
[Abstract] The host microenvironment plays a prominent role in tumor growth, angiogenesis, invasion, metastasis, and response to therapy. Orthotopic tumor model mimics the natural environment of tumor development and provides an effective approach to investigate tumor pathophysiology and develop therapeutic strategies. This protocol describes the technique ...
Pituitary Isograft Transplantation in Mice
[Abstract] The mouse pituitary isograft is a technique developed to administer persistent hormone stimulation, thereby increasing cellular proliferation in the mammary tissue (Christov et al., 1993). The pituitary isograft procedure was first described in ‘Induction of Mammary Cancer in Mice without the Mammary Tumor Agent by Isografts of ...
High Fat Diet-induced Breast Cancer Model in Rat
Authors: Meng Ju Wu and
Chun Ju Chang,
date: 07/05/2016,
view: 5608,
Q&A: 0
[Abstract] Obesity has been linked to breast cancer progression but the underlying mechanisms remain obscure. Being overweight or obese for a woman at the time she is diagnosed with breast cancer is linked to a high risk of recurrence regardless of treatment factors. In rodents, high body weight is also associated with increased incidence of spontaneous and ...
Isolation of Mammary Epithelial Cells and Fibroblasts From Mouse Tumor
Author: Shiva Kazerounian,
date: 02/20/2014,
view: 15881,
Q&A: 0
[Abstract] Developing cancer therapeutics requires the ability to investigate their effects using in vitro models of a specific type of tumor. This protocol provides a method for the isolation and adoption to growth in culture of cells from primary tumors. This is particularly valuable for studying mouse models where original tumor cells can be ...