Measuring Procaspase-8 and -10 Processing upon Apoptosis Induction
[Abstract] Apoptosis or programmed cell death is important for multicellular organisms to keep cell homeostasis and for the clearance of mutated or infected cells. Apoptosis can be induced by intrinsic or extrinsic stimuli. The first event in extrinsic apoptosis is the formation of the Death-Inducing Signalling Complex (DISC), where the initiator caspases-8 ...
Phagocytosis Assay to Measure Uptake of Necroptotic Cancer Cells by BMDCs
[Abstract] This protocol is a flow cytometry-based method to measure the phagocytosis efficiency of necroptotic target cells by bone marrow-derived dendritic cells (BMDCs) in vitro (Aaes et al., 2016). The method is a slightly modified and updated version of the protocols used in previously published papers (Krysko et al., 2006; ...
Senescence Associated β-galactosidase Staining
Authors: Michael Eccles and
Caiyun Grace Li,
date: 08/20/2012,
view: 53530,
Q&A: 14
[Abstract] Detection of senescent cells using a cytochemical assay was first described in 1995 (Dimri et al., 1995). The identification of senescent cells is based on an increased level of lysosomal β-galactosidase activity (Kurz et al., 2000). Cells under normal growth condition produce acid lysosomal β- galactosidase, which is localized ...
Cell Survival Rate Assay
Author: FengZhi Liu,
date: 06/20/2012,
view: 13587,
Q&A: 0
[Abstract] This protocol utilizes PicoGreen 96-well plate technology. This method is applied to estimate the sensitivity of different tumor cell lines to chemodrugs.
Clonogenic Assay
Author: Xiaodong Yang,
date: 05/20/2012,
view: 83337,
Q&A: 5
[Abstract] Clonogenic assays serve as a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumor cells. A colony is defined as a cluster of at least 50 cells that can often only be determined microscopically. A clonogenic assay is the method of choice to determine cell reproductive death after treatment with ionizing ...