Ex vivo Culture Assay Using Human Hair Follicles to Study Circadian Characteristics
[Abstract] Ex vivo culture assays of biopsy specimens are advantageous for the experimental evaluation of human circadian characteristics. We developed a simple and non-invasive experimental evaluation method for monitoring the expression of circadian clock genes in an ex vivo culture assay using human hair follicles. This method imposes ...
Assembly of Genetic Circuits with the Mammalian ToolKit
[Abstract] The ability to rapidly assemble and prototype cellular circuits is vital for biological research and its applications in biotechnology and medicine. The Mammalian ToolKit (MTK) is a Golden Gate-based cloning toolkit for fast, reproducible and versatile assembly of large DNA vectors and their implementation in mammalian models. The MTK consists of ...
Approaching RNA-seq for Cell Line Identification
Authors: Tabrez A. Mohammad and
Yidong Chen,
date: 02/05/2020,
view: 1187,
Q&A: 0
[Abstract] Cancer cell lines serve as invaluable model systems for cancer biology research and help in evaluating the efficacy of new therapeutic agents. However, cell line contamination and misidentification have become one of the most pressing problems affecting biomedical research. Available methods of cell line authentication suffer from limited access, ...
Microarray, IPA and GSEA Analysis in Mice Models
[Abstract] This protocol details a method to analyze two tissue samples at the transcriptomic level using microarray analysis, ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA). Methods such as these provide insight into the mechanisms underlying biological differences across two samples and thus can be applied to interrogate a variety ...
RNA Interference Screening to Identify Proliferation Determinants in Breast Cancer Cells
Authors: Yong-Wei Zhang,
Rochelle E. Nasto,
Sandra A. Jablonski,
Ilya G Serebriiskii,
Rishi Surana,
Joseph Murray,
Michael Johnson,
Rebecca B. Riggins,
Robert Clarke,
Erica A. Golemis and
Louis M. Weiner,
date: 08/05/2017,
view: 5279,
Q&A: 0
[Abstract] RNAi screening technology has revealed unknown determinants of various biological signaling pathways in biomedical studies. This protocol provided detailed information about how to use RNAi screening to identify proliferation determinants in breast tumor cells. siRNA-based libraries targeting against Estrogen receptor (ER)-network, including 631 ...