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Protocol for Measuring Free (Low-stress) Exploration in Rats

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Microarray, IPA and GSEA Analysis in Mice Models

Authors: Stephanie N. Oprescu, Katharine A. Horzmann, Feng Yue, Jennifer L. Freeman and Shihuan Kuang, date: 09/05/2018, view: 3669, Q&A: 0

[Abstract] This protocol details a method to analyze two tissue samples at the transcriptomic level using microarray analysis, ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA). Methods such as these provide insight into the mechanisms underlying biological differences across two samples and thus can be applied to interrogate a variety ...

RNA Interference Screening to Identify Proliferation Determinants in Breast Cancer Cells

Authors: Yong-Wei Zhang, Rochelle E. Nasto, Sandra A. Jablonski, Ilya G Serebriiskii, Rishi Surana, Joseph Murray, Michael Johnson, Rebecca B. Riggins, Robert Clarke, Erica A. Golemis and Louis M. Weiner, date: 08/05/2017, view: 4534, Q&A: 0

[Abstract] RNAi screening technology has revealed unknown determinants of various biological signaling pathways in biomedical studies. This protocol provided detailed information about how to use RNAi screening to identify proliferation determinants in breast tumor cells. siRNA-based libraries targeting against Estrogen receptor (ER)-network, including 631 ...

Synthetic Lethality Screens Using RNAi in Combination with CRISPR-based Knockout in Drosophila Cells

Authors: Benjamin E. Housden, Hilary E. Nicholson and Norbert Perrimon, date: 02/05/2017, view: 6564, Q&A: 0

[Abstract] A synthetic lethal interaction is a type of genetic interaction where the disruption of either of two genes individually has little effect but their combined disruption is lethal. Knowledge of synthetic lethal interactions can allow for elucidation of network structure and identification of candidate drug targets for human diseases such as cancer. ...

Lentiviral shRNA Screen to Identify Epithelial Integrity Regulating Genes in MCF10A 3D Culture

Authors: Elsa Marques and Juha Klefström, date: 12/05/2016, view: 6256, Q&A: 0

[Abstract] MCF10A 3D culture system provides a reductionist model of glandular mammary epithelium which is widely used to study development of glandular architecture, the role of cell polarity and epithelial integrity in control of epithelial cell functions, and mechanisms of breast cancer. Here we describe how to use shRNA screening approach to identify ...

Microinjection of Virus into Lumbar Enlargement of Spinal Dorsal Horn in Mice

Authors: Zhi-Jun Zhang, Peng-Bo Jing and Yong-Jing Gao, date: 11/20/2016, view: 9038, Q&A: 0

[Abstract] In order to explore the role of a specific gene/protein in the specific segment of the spinal cord, the technique of intraspinal injection is particularly used to deliver viral vectors targeting the specific gene/protein. These viral vectors can knockdown or overexpress the specific gene/protein in specific cells (glial cells or neurons). In this ...

Detection of Mitogen-activated Protein Kinase (MAPK) Activation upon Exogenous Chemical Application in Arabidopsis Protoplasts

Authors: Arsheed Hussain Sheikh and Alok Krishna Sinha, date: 09/20/2015, view: 7586, Q&A: 0

[Abstract] The mitogen activated protein kinase cascade is a highly conserved signal pathway in plants. The exogenous chemicals, like hormones, can trigger a series of signalling cascades, including MAPK pathway, to modulate the plant physiology. Upon activation, some MAPKs are phosphorylated. It is important to develop methods that can detect changes in the ...

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