In vitro Demonstration and Quantification of Neutrophil Extracellular Trap Formation
[Abstract] In the recent decade, neutrophil extracellular traps (NETs) have been identified and confirmed as a new anti-microbial weapon of neutrophils. In this protocol, we describe easy methods to demonstrate NET formation by immunofluorescence staining of extracellular chromatin fiber with anti-DNA/Histone H1 antibody and quantification of NETs by using a ...
Visualization of ex vivo Neutrophil Extracellular Traps by Fluorescence Microscopy
Authors: Koji Tanaka,
Tadanobu Shimura,
Yuji Toiyama,
Yoshinaga Okugawa,
Yasuhiro Inoue,
Toshimitsu Araki,
Yasuhiko Mohri,
Akira Mizoguchi and
Masato Kusunoki,
date: 08/05/2015,
view: 8247,
Q&A: 0
[Abstract] Neutrophil extracellular traps (NETs) are extracellular DNAs decorated with nuclear and granular proteins such as histones, neutrophil elastase or myeloperoxidase. They exhibit fibrous mesh-like, web-like, or string-like structures. Here, we describe our protocol regarding visualization of ex vivo NETs released from neutrophils activated ...
Quantification of ex vivo Neutrophil Extracellular Traps
Authors: Koji Tanaka,
Masato Okigami,
Yuji Toiyama,
Yoshinaga Okugawa,
Yasuhiro Inoue,
Toshimitsu Araki,
Yasuhiko Mohri,
Akira Mizoguchi and
Masato Kusunoki,
date: 08/05/2015,
view: 8236,
Q&A: 0
[Abstract] Neutrophil extracellular traps (NETs) are fibrous mesh-like, web-like, or string-like structures which are composed of DNA, histones, and granule proteins such as neutrophil elastase or myeloperoxidase. When activated by phorbol myristate acetate, interleukin-8, lipopolysaccharide (LPS), and various pathogens, neutrophils release NETs. We reported ...