Plant Science


    Protocols in Current Issue
    Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
    Authors:  Oded Skaliter , Jasmin Ravid, Alon Cna'ani, Gony Dvir, Rafael Knafo and Alexander Vainstein, date: 07/05/2018, view: 1594, Q&A: 0
    [Abstract] Plant vacuoles are the largest compartment in plant cells, occupying more than 80% of the cell volume. A variety of proteins, sugars, pigments and other metabolites are stored in these organelles (Paris et al., 1996; Olbrich et al., 2007). Flowers produce a variety of specialized metabolites, some of which are unique to this ...
    Metabolite Profiling of Mature Arabidopsis thaliana Seeds Using Gas Chromatography-Mass Spectrometry (GC-MS)
    Authors:  Hagai Cohen, Ifat Matityahu and Rachel Amir, date: 11/05/2016, view: 5707, Q&A: 0
    [Abstract] Metabolite profiling using gas chromatography-mass spectrometry (GC-MS) permits the annotation and quantification of a relatively wide variety of metabolites, covering a wide range of biochemical groups of metabolites. Lisec et al. (2006) established a method for GC-MS profiling in plants. Based on this protocol, we provide here a ...
    Extraction and Measurement of Strigolactones in Sorghum Roots
    Authors:  Kaori Yoneyama, Xiaonan Xie, Takahito Nomura and Koichi Yoneyama, date: 03/20/2016, view: 5868, Q&A: 0
    [Abstract] Strigolactones (SLs) are carotenoid-derived signaling chemicals containing two lactone moieties in their structures and induce seed germination of root parasitic plants, Striga and Orobanche spp. In the rhizosphere, SLs are essential host recognition signals not only for root parasitic plants but also for arbuscular mycorrhizal ...
    Extraction of Apoplastic Wash Fluids and Leaf Petiole Exudates from Leaves of Arabidopsis thaliana
    Authors:  Takao Araya, Anne Bohner and Nicolaus von Wirén, date: 12/20/2015, view: 7613, Q&A: 0
    [Abstract] The long-distance translocation of metabolites and mineral elements is crucial for plant growth and reproduction. In most cases, source-to-sink translocation of metabolites and minerals requires their passage through the apoplast, irrespective whether they are transported via the xylem or the phloem. This apoplast-mediated pathway is of particular ...
    GC-MS-Based Analysis of Chloroform Extracted Suberin-Associated Root Waxes from Arabidopsis and Other Plant Species
    Authors:  Dylan K. Kosma, Isabel Molina and Owen Rowland, date: 12/20/2015, view: 5828, Q&A: 0
    [Abstract] The periderm and exodermis of taproots and tuberous taproots contain an extracellular lipid polymer, suberin, deposited in their cell walls. This polymer is intractable in organic solvents, and is co-deposited with chloroform-extractable waxes. These suberin-associated root waxes are typically composed of alkanes, primary alcohols, fatty acids, ...
    Isolation and Characterization Procedure for Indole Alkaloids from the Marquesan Plant Rauvolfia Nukuhivensis
    Authors:  Nicolas J. Martin, Maël Nicolas, Gaël Lecellier and Phila Raharivelomanana, date: 10/20/2015, view: 6057, Q&A: 0
    [Abstract] A plethora of natural products, mostly secondary metabolites, are isolated and purified from many different organisms, like plants, fungi, algae, marine invertebrates, etc. The extraction procedure is specific to each organism, but some guidelines are usually followed for any purification procedure regarding targeted metabolites, such as ...
    Arabidopsis Metabolome Analysis Using Infusion ESI FT-ICR/MS
    Authors:  Reiko Motohashi, Masakazu Satou, Fumiyoshi Myouga, Akira Oikawa and Daisaku Ohta, date: 05/05/2015, view: 4909, Q&A: 0
    [Abstract] We made the method for Arabidopsis metabolome analysis based on direct-infusion Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS) (IonSpec). This method was sufficiently applied to metabolic phenotyping of Arabidopsis. This method is simple in that after homogenizing samples, powdered samples are dissolved ...

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