Plant Science

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    Protocols in Current Issue
    Quantification of Starch in Guard Cells of Arabidopsis thaliana
    Authors:  Sabrina Flütsch, Luca Distefano and Diana Santelia, date: 07/05/2018, view: 4545, Q&A: 0
    [Abstract] In this protocol, we describe how to quantify starch in guard cells of Arabidopsis thaliana using the fluorophore propidium iodide and confocal laser scanning microscopy. This simple method enables monitoring, with unprecedented resolution, the dynamics of starch in guard cells.
    An Evaluation of Cellulose Degradation Affected by Dutch Elm Disease
    Authors:  Jaroslav Ďurkovič, František Kačík and Dušan Olčák, date: 07/20/2015, view: 6232, Q&A: 0
    [Abstract] The pathogenic fungus Ophiostoma novo-ulmi spreads within the secondary xylem vessels of infected elm trees, causing the formation of vessel plugs due to tyloses and gels, which ultimately result in Dutch elm disease. Foliage discoloration, wilting and falling from the tree are typical external leaf symptoms of the disease followed by the ...
    Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
    [Abstract] Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch ...
    Determination of Soluble Sugars in Arabidopsis thaliana Leaves by Anion Exchange Chromatography
    Authors:  M. Isabel Ortiz-Marchena, M. Teresa Ruiz, Federico Valverde and José M. Romero, date: 12/05/2014, view: 10595, Q&A: 0
    [Abstract] Determination of soluble sugars is basic for the study of carbon metabolism in plants. Soluble sugar quantitation can be achieved by enzymatic methods implying different coupled reactions. Here we describe a simple method that allows rapid determination of the most abundant soluble sugars (glucose, fructose and sucrose) in Arabidopsis ...



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