Biochemistry

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    Protocols in Current Issue
    Assaying Glycogen and Trehalose in Yeast
    Authors:  Yuping Chen and Bruce Futcher, date: 07/05/2017, view: 4236, Q&A: 0
    [Abstract] Organisms store carbohydrates in several forms. In yeast, carbohydrates are stored in glycogen (a multi-branched polysaccharide) and in trehalose (a disaccharide). As in other organisms, the amount of stored carbohydrate varies dramatically with physiological state, and accordingly, an assay of stored carbohydrate can help reveal physiological ...
    Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition
    Authors:  Susan M. Carnachan and Simon F.R. Hinkley, date: 04/05/2017, view: 4422, Q&A: 0
    [Abstract] Heparan sulfate (HS) is purified from complex matrices and often not fully characterised to validate its assignment. The characterisation of heparins and heparan sulfates through enzymatic depolymerisation and subsequent strong anion-exchange high performance liquid chromatography (SAX-HPLC) analysis and quantitation of the resulting disaccharides ...
    Heparan Sulfate Identification and Characterisation: Method I. Heparan Sulfate Identification by NMR Analysis
    Authors:  Susan M. Carnachan and Simon F.R. Hinkley, date: 04/05/2017, view: 4014, Q&A: 0
    [Abstract] Heparin and heparan sulfate (HS) may be purified from complex biological matrices and are often isolated in sub-milligram quantities but not unequivocally identified by spectroscopic means. This protocol details a methodology to rapidly assess the gross compositional features and approximate purity of HS by 1H nuclear magnetic ...
    Trehalase Activity in Arabidopsis thaliana Optimized for 96-well Plates
    Authors:  Hilde Van Houtte and Patrick Van Dijck, date: 10/20/2013, view: 7153, Q&A: 1
    [Abstract] Trehalose is a nonreducing disaccharide. It is a common sugar in bacteria, fungi and yeast, where it functions as a carbon source and stress protectant. In contrast, plants, although encoding large trehalose biosynthesis gene families, contain only small amounts of trehalose. The intermediate compound of trehalose, trehalose-6-phosphate (T6P), is ...



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