Cell Biology

Categories

    Protocols in Current Issue
    Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
    Authors:  Yanrong You, Chunpeng An and Chuanyou Li, date: 03/05/2020, view: 716, Q&A: 0
    [Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...
    Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling
    Authors:  Yan Bao and Diane C. Bassham, date: 12/05/2018, view: 2774, Q&A: 0
    [Abstract] Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein response (UPR). Assays for activation of the UPR in plants include Tunicamycin (Tm)- or dithiothreitol ...
    Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea
    Authors:  Giovanna Salbitani, Claudia Bottone and Simona Carfagna, date: 07/05/2017, view: 12042, Q&A: 0
    [Abstract] Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved ...
    P-body and Stress Granule Quantification in Caenorhabditis elegans
    Authors:  Matthias Rieckher and Nektarios Tavernarakis, date: 01/20/2017, view: 8248, Q&A: 0
    [Abstract] Eukaryotic cells contain various types of cytoplasmic, non-membrane bound ribonucleoprotein (RNP) granules that consist of non-translating mRNAs and a versatile set of associated proteins. One prominent type of RNP granules is Processing bodies (P bodies), which majorly harbors translationally inactive mRNAs and an array of proteins mediating mRNA ...
    In vitro Treatment of Mouse and Human Cells with Endogenous Ligands for Activation of the Aryl Hydrocarbon Receptor
    Authors:  Taisho Yamada and Akinori Takaoka, date: 01/05/2017, view: 5150, Q&A: 0
    [Abstract] Activation of the aryl hydrocarbon receptor (AHR) by endogenous ligands has been implicated in a variety of physiological processes such as cell cycle regulation, cell differentiation and immune responses. It is reported that tryptophan metabolites, such as kynurenine (Kyn) and 6-formylindolo(3,2-b)carbazole (FICZ), are endogenous ligands for AHR ...
    FICZ Exposure and Viral Infection in Mice
    Authors:  Taisho Yamada and Akinori Takaoka, date: 01/05/2017, view: 5359, Q&A: 0
    [Abstract] The aryl hydrocarbon receptor (AHR) is known as a sensor for dioxins that mediates their toxicity, and also has important biophysiological roles such as circadian rhythms, cell differentiation and immune responses. 6-formylindolo(3,2-b)carbazole (FICZ), which is derived through the metabolism of L-tryptophan by ultraviolet B irradiation, is one of ...
    In vivo Analysis of Neutrophil Infiltration during LPS-induced Peritonitis
    Authors:  Lucia de Almeida, Andrea Dorfleutner and Christian Stehlik, date: 10/05/2016, view: 6813, Q&A: 0
    [Abstract] Bacterial lipopolysaccharide (LPS) is present in the outer membrane of Gram-negative bacteria and functions as pathogen-associated molecular pattern (PAMP) (Whitfield and Trent, 2014). LPS therefore is a potent activator of inflammatory responses leading to cytokine release and neutrophils recruitment. The lipid A moiety of LPS activates the ...
    ASC-particle-induced Peritonitis
    Authors:  Lucia de Almeida, Andrea Dorfleutner and Christian Stehlik, date: 10/05/2016, view: 4458, Q&A: 0
    [Abstract] In response to pathogen infection and tissue damage, inflammasome sensors such as NLRP3 and AIM2 are activated, which triggers PYRIN domain (PYD)-mediated ASC nucleation, followed by self-perpetuating ASC polymerization, which ultimately culminates in caspase-1 activation, interleukin (IL)-1β and IL-18 processing and release and pyroptosis ...
    Hepatitis C virus Cell-to-cell Spread Assay
    Authors:  Naina Barretto and Susan L. Uprichard, date: 12/20/2014, view: 7831, Q&A: 1
    [Abstract] Hepatitis C virus (HCV) can infect naïve cells via entry of “cell-free” extracellular virus or direct “cell-to-cell” transmission. Here, we describe an assay for detecting HCV cell-to-cell transmission, using a non-growing cell culture system that avoids confounding effects of cell growth. The assay consists of infecting a small number of cells in ...
    Signaling Assays for Detection of Human G-protein-coupled Receptors in Yeast
    Authors:  Yasuyuki Nakamura, Jun Ishii and Akihiko Kondo, date: 08/20/2014, view: 7916, Q&A: 0
    [Abstract] G-protein-coupled receptors (GPCRs) are the largest group of cell-surface proteins and are major molecular targets for drug development. The protocol described herein is for the detection of human GPCR signaling in the yeast Saccharomyces cerevisiae. Using Zoanthus sp. green fluorescent protein (ZsGreen) as the reporter, ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.