Single-cell Motility Analysis of Tethered Human Spermatozoa
[Abstract] Vigorous sperm flagellar motility is essential for fertilization, and so the quantitative measurement of motility is a useful tool to assess the intrinsic fertility potential of sperm cells and explore how various factors can alter sperm’s ability to reach the egg and penetrate its protective layers. Human sperm beat their flagella many times each ...
Bacterial Aggregation Assay in the Presence of Cyclic Lipopeptides
Authors: Pengyuan Xiu,
Rui Liu,
Dechao Zhang and
Chaomin Sun,
date: 01/05/2018,
view: 2821,
Q&A: 0
[Abstract] Lipopeptides is an important class of biosurfactants having antimicrobial and anti-adhesive activity against pathogenic bacteria. These include surfactin, fengycin, iturin, bacillomycin, mycosubtilin, lichenysin, and pumilacidin (Arima et al., 1968; Naruse et al., 1990; Yakimov et al., 1995; Steller and Vater, 2000; ...
Plasmodium Sporozoite Motility on Flat Substrates
[Abstract] Plasmodium sporozoites are the infectious, highly motile forms of the malaria parasite transmitted by Anopheles mosquitoes. Sporozoite motility can be assessed following the dissection of Anopheles salivary glands and isolation of sporozoites in vitro.
ARP2/3 Phosphorylation Assay in the Presence of Recombinant Bacterial Effectors
[Abstract] The Actin-Related Protein 2/3 (ARP2/3) complex is an actin nucleator that generates a branched actin network in mammalian cells. In addition to binding nucleation promoting factors, LeClaire et al. demonstrated that its phosphorylation state is essential key for its activity (LeClaire et al., 2008). In cells, the ARP2/3 complex ...
Measurements of Free-swimming Speed of Motile Salmonella Cells in Liquid Media
[Abstract] Bacteria such as Escherichia coli and Salmonella enterica swim in liquid media using the bacterial flagella. The flagellum consists of the basal body (rotary motor), the hook (universal joint) and the filament (helical screw). Since mutants with a defect in flagellar assembly and function cannot swim smoothly, motility assay is ...
Bone Marrow Mesenchymal Stem Cells Adhesion Assay
Authors: Zhigang Yang and
Ran Xiao,
date: 08/05/2016,
view: 5843,
Q&A: 0
[Abstract] Mesenchymal stem cells (MSCs) are widespread in adult organisms and involved in tissue maintenance and repair as well as in the regulation of hematopoiesis and immunologic responses. As cell adhesion play important roles in cell interactions and signaling, to thoroughly evaluate the adhesion ability of MSCs is of vital importance to clarify the ...
Three Dimensional Spheroid Co-culture Invasion Assay
Authors: Eldo T Verghese and
Thomas A Hughes,
date: 01/05/2015,
view: 8574,
Q&A: 0
[Abstract] The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells (in our case breast cancer cell lines) (Verghese et al., 2013). Typical two dimensional ...