Neuroscience

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    Protocols in Current Issue
    Artificial Inhalation Protocol in Adult Mice
    Authors:  Thomas P. Eiting and Matt Wachowiak, date: 09/20/2018, view: 1040, Q&A: 0
    [Abstract] Research in the area of in vivo olfactory physiology benefits from having direct access to the nasal airways through which odorants can be presented. Ordinarily, the passage of odorants through the airways is controlled by respiratory rhythm. This fact makes it difficult to control the timing and strength of an olfactory stimulus, since ...
    In vivo Use of Dextran-based Anterograde Cortical Tracers to Assess the Integrity of the Cortical Spinal Tract
    Authors:  Anna M. Wiersma and Ian R. Winship, date: 05/20/2018, view: 1857, Q&A: 0
    [Abstract] When injected into the motor cortex of rats, anterograde tracers label fibers of the associated descending corticospinal tract (CST) that originate from pyramidal neurons in the tracer-injected cortex. These fibers can be assessed at the level of the spinal cord to determine the integrity of the descending CST and the spatial distribution of axons ...
    zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain
    [Abstract] In studies of brain function, it is essential to understand the underlying neuro-architecture. Very young zebrafish larvae are widely used for neuroarchitecture studies, due to their size and natural transparency. However, this model system has several limitations, due to the immaturity, high rates of development and limited behavioral repertoire ...
    A Streamlined Method for the Preparation of Gelatin Embedded Brains and Simplified Organization of Sections for Serial Reconstructions
    Authors:  Andrew W. Liu, Sho Aoki and Jeffery R. Wickens, date: 11/20/2017, view: 4236, Q&A: 0
    [Abstract] Gelatin embedding of whole brains for sectioning is a critical procedure used in neuroscience to ensure all morphological and spatial details are preserved intact. Here, we describe an inexpensive, reproducible and efficient means to embed post-fixed brains ready for sectioning in gelatin within a week’s time. The sections obtained are ...
    Stereotaxic Surgery for Suprachiasmatic Nucleus Lesions in Mice
    Authors:  Kimiko Shimizu and Yoshitaka Fukada, date: 06/20/2017, view: 7786, Q&A: 0
    [Abstract] Site-specific lesions are invaluable methods for investigating the function of brain regions within the central nervous system and can be used to study neural mechanisms of behaviors. Precise stereotaxic surgery is required to lesion small regions of the brain such as the suprachiasmatic nucleus (SCN), which harbors the master circadian clock. In ...
    Extracellular Axon Stimulation
    Authors:  Carola Städele, Margaret Louise DeMaegd and Wolfgang Stein, date: 03/05/2017, view: 5488, Q&A: 0
    [Abstract] This is a detailed protocol explaining how to perform extracellular axon stimulations as described in Städele and Stein, 2016. The ability to stimulate and record action potentials is essential to electrophysiological examinations of neuronal function. Extracellular stimulation of axons traveling in fiber bundles (nerves) is a classical technique ...
    Assessment of Mechanical Allodynia in Rats Using the Electronic Von Frey Test
    Authors:  Jeremy Ferrier, Fabien Marchand and David Balayssac, date: 09/20/2016, view: 10705, Q&A: 0
    [Abstract] Chronic pain is one of the most debilitating conditions, affecting one out of five people worldwide. Preclinical models in rodents represent a valuable tool for the study of pathophysiological mechanisms and the discovery of new analgesic drugs. However, pain evaluation in rodents is rather challenging. Altered response to mechanical or thermal ...
    Mouse Auditory Brainstem Response Testing
    Authors:  Omar Akil, A. E. Oursler, Kevin Fan and Lawrence R. Lustig, date: 03/20/2016, view: 8973, Q&A: 0
    [Abstract] The auditory brainstem response (ABR) test provides information about the inner ear (cochlea) and the central pathways for hearing. The ABR reflects the electrical responses of both the cochlear ganglion neurons and the nuclei of the central auditory pathway to sound stimulation (Zhou et al., 2006; Burkard et al., 2007). The ABR ...
    Measuring Blood-brain-barrier Permeability Using Evans Blue in Mice
    Authors:  Jun-Xia Yang, Yan-Yu Jiang and Yu-Bai Guo, date: 08/05/2015, view: 12159, Q&A: 1
    [Abstract] The blood–brain barrier (BBB) is a highly selective permeability barrier that separates the circulating blood from the brain extracellular fluid in the central nervous system. The blood–brain barrier allows the passage of water, some gases, and lipid soluble molecules by passive diffusion, as well as the selective transport of molecules such as ...
    In utero Electroporation of the Embryonic Mouse Retina
    Authors:  Francisco Nieto-Lopez and Luisa Sanchez-Arrones, date: 10/05/2014, view: 9617, Q&A: 0
    [Abstract] This protocol is useful to manipulate gene expression in the embryonic retina and compare the result with the contralateral non electroporated retina. In addition, the electroporation of a membrane or cytoplasmic tagged GFP allows to determine the effects of gene manipulation on the outgrowth of retinal ganglion cell axons (Garcia-Frigola et al. ...



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