Microbiology

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    Protocols in Current Issue
    In vitro Autophosphorylation and Phosphotransfer Assay of Cyanobacterial Histidine Kinase 2
    Author:  Iskander M. Ibrahim, date: 12/05/2016, view: 7476, Q&A: 0
    [Abstract] This is a detailed protocol of an autophosphorylation and phosphotransfer activities of Synechocystis sp. PCC 6803 full-length Histidine Kinase 2 (Hik2) protein described by Ibrahim et al., 2016. In this protocol, radioactively labelled ATP was used to study an autophosphorylation and phosphotransfer activity of the full-length ...
    Detection of Hog1 Phosphorylation in Candida albicans in Response to an Antifungal Protein
    Authors:  Brigitte ME Hayes and Nicole L van der Weerden, date: 09/20/2014, view: 6787, Q&A: 0
    [Abstract] It is becoming increasingly apparent that stress signalling is important for tolerance of fungal species to antifungal chemicals and proteins. The high-osmolarity glycerol (HOG) pathway responds to a number of stressors including osmotic and oxidative stress. This protocol describes a method to detect activation of the Candida albicans ( ...
    Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
    Authors:  Qing Chen, Alice Boulanger, Deborah M. Hinton and Scott Stibitz, date: 11/20/2013, view: 8655, Q&A: 0
    [Abstract] Protein phosphorylation plays a central role in signal transduction in bacteria. However, separation and detection of the phosphorylated protein from its nonphosphorylated form remain challenging. Here we describe a method to detect phosphorylation of the Bordetella pertussis response regulator BvgA, which is phosphorylated at an ...



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