Developmental Biology

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    Protocols in Current Issue
    Activation of Fibroblast Contractility via Cell-Cell Interactions and Soluble Signals
    Authors:  Neha Pincha, Dyuti Saha, Tanay Bhatt, Ravindra K. Zirmire and Colin Jamora, date: 09/20/2018, view: 2345, Q&A: 0
    [Abstract] The collagen contraction assay is an in vitro, three-dimensional method to determine the factor(s) affecting the contractile behavior of activated cells such as fibroblasts in either physiological or pathological scenarios. The collagen lattices/hydrogels are seeded with fibroblasts to mimic the interactions between these cells and their ...
    Muscle Function Assessment Using a Drosophila Larvae Crawling Assay
    Authors:  Yanina Post and Achim Paululat, date: 07/20/2018, view: 2198, Q&A: 0
    [Abstract] Here we describe a simple method to measure larval muscle contraction and locomotion behavior. The method enables the user to acquire data, without the necessity of using expensive equipment (Rotstein et al., 2018). To measure contraction and locomotion behaviour, single larvae are positioned at the center of a humidified Petri dish. ...
    Fluorescence Microscopy for Cilia in Cultured Cells and Zebrafish Embryos
    Authors:  Jingli Cao, Xueliang Zhu and Xiumin Yan, date: 07/20/2014, view: 10489, Q&A: 0
    [Abstract] Cilia are microtubule-based hair-like projections found in organisms, ranging from protozoa to mammals. This protocol provides methods for immunofluorescence staining of cilia in cultured cells and zebrafish embryos.
    In situ Chemotaxis Assay in Caenorhabditis elegans (for the Study of Circadian Rhythms)
    Authors:  Martha Merrow and Maria Olmedo, date: 02/05/2014, view: 9709, Q&A: 0
    [Abstract] Olfaction is a well-studied sensory mechanism in Caenorhabditis elegans (C. elegans). The nematodes respond to a wide range of chemicals by either attraction, repulsion or a mixture thereof (Bargmann et al., 1993). We have used olfaction to characterize behavioural and molecular circadian rhythms in C. elegans. ...
    Acquisition of Leftward Flow in Xenopus laevis
    Authors:  Thomas Thumberger and Martin Blum, date: 12/05/2013, view: 6253, Q&A: 0
    [Abstract] In Xenopus, the left-right axis is established following an extracellular vectorial leftward flow driven by monocilia at the gastrocoel roof plate (GRP) during late gastrulation / neurulation (Schweickert et al., 2007). As the GRP lies inside the developing archenteron, imaging of flow is challenging. Here we present the detailed ...
    Motility Assay for Zebrafish Embryos
    Authors:  Michelle F. Goody and Clarissa A. Henry, date: 06/05/2013, view: 8658, Q&A: 0
    [Abstract] Analyzing the swimming ability of 2 days post fertilization zebrafish embryos can be a useful technique to study neuromuscular function. Here is a protocol for determining the time it takes for zebrafish embryos to swim a predetermined distance.



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