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    Mouse Model of Immune Complex-mediated Vasculitis in Dorsal Skin and Assessment of the Neutrophil-mediated Tissue Damage
    [Abstract] Neutrophils are the most abundant leukocytes in the blood. In the recent decades, their crucial roles in host defense, immune regulation and tissue damage have been studied in a deeper dimension. In this protocol, we described a mouse model of immune complex-mediated vasculitis in the dorsal skin induced by Arthus reaction, and the subsequent ...
    zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain
    [Abstract] In studies of brain function, it is essential to understand the underlying neuro-architecture. Very young zebrafish larvae are widely used for neuroarchitecture studies, due to their size and natural transparency. However, this model system has several limitations, due to the immaturity, high rates of development and limited behavioral repertoire ...
    Histochemical Staining of Collagen and Identification of Its Subtypes by Picrosirius Red Dye in Mouse Reproductive Tissues
    [Abstract] Collagen is one of the foremost components of tissue extracellular matrix (ECM). It provides strength, elasticity and architecture to the tissue enabling it to bear the wear and tear from external factors like physical stress as well as internal stress factors like inflammation or other pathological conditions. During normal pregnancy or pregnancy ...
    Mouse Model of Dextran Sodium Sulfate (DSS)-induced Colitis
    Authors:  Srustidhar Das, Surinder K. Batra and Satyanarayana Rachagani, date: 08/20/2017, view: 1433, Q&A: 0
    [Abstract] Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the intestinal tract and is primarily comprised of Crohn’s disease (CD) and ulcerative colitis (UC). Several murine models that include both chemical induced and genetic models have been developed that mimic some aspects of either CD or UC. These models have been instrumental in ...
    Isolation of Ustilago bromivora Strains from Infected Spikelets through Spore Recovery and Germination
    Authors:  Jason Bosch and Armin Djamei, date: 07/20/2017, view: 1028, Q&A: 0
    [Abstract] Ustilago bromivora is a biotrophic smut fungus infecting Brachypodium sp. It is closely related to the barley-infecting smut Ustilago hordei, and related to the well-studied, gall-inducing model pathogen Ustilago maydis. Upon flowering, the spikelets of U. bromivora-infected plants are filled with black ...
    Analysis of 3D Cellular Organization of Fixed Plant Tissues Using a User-guided Platform for Image Segmentation
    Authors:  Ethel Mendocilla Sato and Célia Baroux, date: 06/20/2017, view: 1588, Q&A: 0
    [Abstract] The advent of non-invasive, high-resolution microscopy imaging techniques and computational pipelines for high-throughput image processing has contributed to gain insights in plant organ morphogenesis at the cellular level. Confocal scanning laser microscopy (CSLM) allows the generation of three dimensional images constituted of serial optical ...
    Whole-seed Immunolabeling of Arabidopsis Mucilage Polysaccharides
    Author:  Cătălin Voiniciuc, date: 06/05/2017, view: 1219, Q&A: 0
    [Abstract] In addition to synthesizing and secreting copious amounts of pectic polymers (Young et al., 2008), Arabidopsis thaliana seed coat epidermal cells produce small amounts of cellulose and hemicelluloses typical of secondary cell walls (Voiniciuc et al., 2015c). These components are intricately linked and are released as a ...
    Muscle Histology Characterization Using H&E Staining and Muscle Fiber Type Classification Using Immunofluorescence Staining
    Authors:  Chao Wang, Feng Yue and Shihuan Kuang, date: 05/20/2017, view: 2628, Q&A: 0
    [Abstract] Muscle function is determined by its structure and fiber type composition. Here we describe a protocol to examine muscle histology and myofiber types using hematoxylin and eosin (H&E) and immunofluorescence staining, respectively. H&E stain nucleus in blue and cytoplasm in red, therefore allowing for morphological analyses, such as myofiber ...
    Penetration Assays, Fungal Recovery and Pathogenicity Assays for Verticillium dahliae
    Authors:  Yun-Long Zhao, Tao Zhang and Hui-Shan Guo, date: 02/20/2017, view: 2250, Q&A: 0
    [Abstract] Verticillium dahliae is a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues. The great loss of economically important crop caused by V. dahliae has raised worldwide concern, however, little is known about the mechanism of its pathogenicity (Klosterman et al., 2011; Yadeta and ...
    Various Modes of Spinal Cord Injury to Study Regeneration in Adult Zebrafish
    Authors:  Subhra Prakash Hui and Sukla Ghosh, date: 12/05/2016, view: 2994, Q&A: 0
    [Abstract] Spinal cord injury (SCI) in mammals leads to failure of both sensory and motor functions, due to lack of axonal regrowth below the level of injury as well as inability to replace lost neural cells and to stimulate neurogenesis. In contrast, fish and amphibians are capable of regenerating a variety of their organs like limb/fin, jaw, heart and ...