Acute Cerebellar Slice Preparation Using a Tissue Chopper
Authors: Yubin Wang and
Michel Baudry,
date: 03/05/2019,
view: 496,
Q&A: 0
[Abstract] Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
Obtaining Acute Brain Slices
Authors: Thomas Papouin and
Philip G. Haydon,
date: 01/20/2018,
view: 5154,
Q&A: 0
[Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...
Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery
[Abstract] In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of ‘gain-of-function’ and ‘loss-of-function’ blastocyst ...
Preparation of Teased Nerve Fibers from Rat Sciatic Nerve
Authors: Jinkun Wen,
Lixia Li,
Dandan Tan and
Jiasong Guo,
date: 10/05/2017,
view: 4079,
Q&A: 0
[Abstract] Compared to tissue sectioning techniques, the technique of teasing single nerve fibers provides a better way to understand the structures of myelin sheaths and axons of the peripheral myelinated nerves. This protocol describes a method for preparation of teased single nerve fibers from rat sciatic nerve. In this protocol, fixed nerves are teased ...
Isolation of Rodent Brain Vessels
Authors: Cristina Carvalho and
Paula I. Moreira,
date: 09/05/2017,
view: 3681,
Q&A: 0
[Abstract] The prevalence of neurodegenerative diseases is increasing worldwide. Cerebrovascular disorders and/or conditions known to affect brain vasculature, such as diabetes, are well-known risk factors for neurodegenerative diseases. Thus, the evaluation of the brain vasculature is of great importance to better understand the mechanisms underlying brain ...