F-actin Bundle Sedimentation Assay
Authors: Shan-Shan Lin,
Mei-Chun Chuang and
Ya-Wen Liu,
date: 11/05/2019,
view: 2347,
Q&A: 0
[Abstract] Understanding the molecular mechanism governing the higher-order regulation of actin dynamics requires chemically-defined and quantitative assays. Recently, the membrane remodeling large GTPase, dynamin, has been identified as a new actin cross-linking molecule. Dynamin regulates actin cytoskeleton through binding to, self-assembling around, and ...
Hydrogen Deuterium Exchange Mass Spectrometry of Oxygen Sensitive Proteins
[Abstract] The protocol detailed here describes a way to perform hydrogen deuterium exchange coupled to mass spectrometry (HDX-MS) on oxygen sensitive proteins. HDX-MS is a powerful tool for studying the protein structure-function relationship. Applying this technique to anaerobic proteins provides insight into the mechanism of proteins that perform oxygen ...