Biochemistry

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    Protocols in Current Issue
    Transient Transfection-based Fusion Assay for Viral Proteins
    Authors:  Melina Vallbracht, Christina Schröter, Barbara G. Klupp and Thomas C. Mettenleiter, date: 03/05/2017, view: 7309, Q&A: 1
    [Abstract] Membrane fusion is vital for entry of enveloped viruses into host cells as well as for direct viral cell-to-cell spread. To understand the fusion mechanism in more detail, we use an infection free system whereby fusion can be induced by a minimal set of the alphaherpesvirus pseudorabies virus (PrV) glycoproteins gB, gH and gL. Here, we describe an ...
    Protein-lipid Interaction Analysis by Surface Plasmon Resonance (SPR)
    Authors:  Olga Lucia Baron and David Pauron, date: 09/20/2014, view: 9679, Q&A: 0
    [Abstract] Interactions of lipids with proteins are essential events in the framework of biological membranes. Assessment of the affinity and specificity of protein-lipid binding can give useful information to elucidate cell membrane functions. Surface Plasmon Resonance (SPR) is a powerful technology to study macromolecular interactions, allowing direct and ...
    Dye Release Experiments with Dextran Loaded Vesicles
    Authors:  Marc-Antoine Sani, Neil M. O’Brien-Simpson and Frances Separovic, date: 07/20/2014, view: 7249, Q&A: 0
    [Abstract] Dye release experiments are a widely used method to assess the interactions between membrane-active molecules and lipid membranes. Of particular interest is the ability to assess the degree of the lipid bilayer perturbation by simultaneously encapsulating dye of different sizes, such as dextrans grafted with a chromophore. In this assay, dextran ...
    In vitro Lipid Binding Assay
    Authors:  Yan Zhang and Xiaochen Wang, date: 05/05/2013, view: 11765, Q&A: 0
    [Abstract] This is a protocol to examine in vitro protein-lipid binding using membrane strips coated with various lipids. It has been successfully used to study in vitro interaction between lipids and C. elegans proteins.



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