Biochemistry

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    Protocols in Current Issue
    Hypochlorous Acid Staining with R19-S in the Drosophila Intestine upon Ingestion of Opportunistic Bacteria
    Authors:  Salma Hachfi, Olivia Benguettat and Armel Gallet, date: 05/20/2019, view: 105, Q&A: 0
    [Abstract] The intestine is endowed with an innate immune system that is required to fight any exogenous bacteria that are swallowed along with the food. The first line of defense that is mounted by the gut epithelium is the release of immune Reactive Oxygen Species (ROS), such as hypochlorous acid (HOCl), into the lumen. HOCl is produced within 1.5 h of ...
    Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-sensing Probe 2’,7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA)
    Authors:  Rajneesh, Jainendra Pathak, Ananya Chatterjee, Shailendra P. Singh and Rajeshwar P. Sinha, date: 09/05/2017, view: 6991, Q&A: 0
    [Abstract] Reactive oxygen species (ROS) are cell signaling molecules synthesized inside the cells as a response to routine metabolic processes. In stress conditions such as ultraviolet radiation (UVR), ROS concentration increases several folds in the cells that become toxic for the cell survival. Here we present the method for in vivo detection of ...
    Measuring Oxidative Stress in Caenorhabditis elegans: Paraquat and Juglone Sensitivity Assays
    Authors:  Megan M. Senchuk, Dylan J. Dues and Jeremy M. Van Raamsdonk, date: 01/05/2017, view: 6794, Q&A: 0
    [Abstract] Oxidative stress has been proposed to be one of the main causes of aging and has been implicated in the pathogenesis of many diseases. Sensitivity to oxidative stress can be measured by quantifying survival following exposure to a reactive oxygen species (ROS)-generating compound such as paraquat or juglone. Sensitivity to oxidative stress is a ...
    Detection of Reactive Oxygen Species in Oryza sativa L. (Rice)
    Authors:  Navdeep Kaur, Isha Sharma, Kamal Kirat and Pratap Kumar Pati, date: 12/20/2016, view: 8361, Q&A: 1
    [Abstract] Superoxide ions (O2-) and hydrogen peroxide (H2O2) are the reactive oxygen species (ROS) that play a significant role in regulation of many plant processes. The level of O2- ions is determined qualitatively using nitrobluetetrazolium (NBT) assay while the H2O2 is qualitatively ...
    Hydrogen Peroxide Measurement in Arabidopsis Root Tissue Using Amplex Red
    Authors:  Tzvetina Brumbarova, Cham Thi Tuyet Le and Petra Bauer, date: 11/05/2016, view: 7499, Q&A: 0
    [Abstract] This protocol describes the measurement of hydrogen peroxide (H2O2) content in Arabidopsis root tissue by using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. When root tissue is disrupted and resuspended in phosphate buffer, H2O2 is released from the cells. The obtained root ...
    In vitro Real-time Measurement of the Intra-bacterial Redox Potential
    Authors:  Joris van der Heijden and B. Brett Finlay, date: 09/05/2015, view: 4860, Q&A: 0
    [Abstract] All bacteria that live in oxygenated environments have to deal with oxidative stress caused by some form of exogenous or endogenous reactive oxygen species (ROS) (Imlay, 2013). Large quantities of ROS damage DNA, lipids and proteins which can eventually lead to bacterial cell death (Imlay, 2013). In contrast, smaller quantities of ROS can play ...
    Electron Paramagnetic Resonance (EPR) Spectroscopy to Detect Reactive Oxygen Species in Staphylococcus aureus
    [Abstract] Under aerobic conditions, Staphylococcus aureus (S. aureus) primarily metabolizes glucose to acetic acid. Although normally S. aureus is able to re-utilize acetate as a carbon source following glucose exhaustion, significantly high levels of acetate in the culture media may not only be growth inhibitory but also ...
    Detection of Alternative Oxidase Expression in Arabidopsis thaliana Protoplasts Treated with Aluminium
    Authors:  Jian Liu, Zhe Li and Da Xing, date: 07/05/2015, view: 5090, Q&A: 0
    [Abstract] Aluminium (Al), a non-essential metal widespread in the environment that is known to be toxic to humans as well as to plants, can cause damage not only to the roots but also to the aerial parts of plants. Its toxicity has been recognized as one of the major factors that limit crop production on acid soil. Alternative oxidase, the respiratory ...
    Chemiluminescence Detection of the Oxidative Burst in Plant Leaf Pieces
    Authors:  Markus Albert, Melinka A. Butenko, Reidunn B. Aalen, Georg Felix and Mari Wildhagen, date: 03/20/2015, view: 10329, Q&A: 1
    [Abstract] The production of 'reactive oxygen species' (ROS), also termed oxidative burst, is a typical cellular response of animals and plants to diverse biotic and abiotic stresses. Here, we outline the detection of the ROS-burst in plant leaf pieces using a luminol-based bioassay which allows for the detection of chemiluminescence. The assay was ...
    Localisation and Quantification of Reactive Oxygen Species and Nitric Oxide in Arabidopsis Roots in Response to Fungal Infection
    Authors:  Kapuganti J. Gupta, Yariv Brotman and Luis A. J. Mur, date: 10/05/2014, view: 7800, Q&A: 0
    [Abstract] Nitric oxide and reactive oxygen species have emerged as important signalling molecules in plants. The half-lives of NO and ROS are very short therefore rapid and precise measurements are required for the understanding biological roles of these redox active species. Various organelles and compartments generate NO and ROS thus it is important to ...



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