Plant Science

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    Protocols in Current Issue
    Analysis of Metals in Whole Cells, Thylakoids and Photosynthetic Protein Complexes in Synechocystis sp. PCC6803
    Authors:  Chiara Gandini, Søren Husted and Sidsel Birkelund Schmidt, date: 06/20/2018, view: 1575, Q&A: 0
    [Abstract] Metals are essential in many biological processes, including oxygenic photosynthesis. Here we described a method to measure the metal pool in whole cells and thylakoids, including the bioactive pool in intact photosynthetic protein complexes in the model oxygenic cyanobacterium Synechocystis PCC6803. In the first part of the protocol, ...
    Evaluation of the Condition of Respiration and Photosynthesis by Measuring Chlorophyll Fluorescence in Cyanobacteria
    Authors:  Takako Ogawa and Kintake Sonoike , date: 05/05/2018, view: 2138, Q&A: 0
    [Abstract] Chlorophyll fluorescence measurements have been widely used to monitor the condition of photosynthesis. Furthermore, chlorophyll fluorescence from cyanobacteria reflects the condition of respiration, since cyanobacterial photosynthesis shares several components of electron transport chain with respiration. This protocol presents the method to ...
    Quantifying the Capacity of Phloem Loading in Leaf Disks with [14C]Sucrose
    Authors:  Umesh P Yadav, Aswad S Khadilkar, Mearaj A Shaikh, Robert Turgeon and Brian G Ayre, date: 12/20/2017, view: 2424, Q&A: 0
    [Abstract] Phloem loading and transport of photoassimilate from photoautotrophic source leaves to heterotrophic sink organs are essential physiological processes that help the disparate organs of a plant function as a single, unified organism. We present three protocols we routinely use in combination with each other to assess (1) the relative rates of ...
    Assessing Long-distance Transport from Photosynthetic Source Leaves to Heterotrophic Sink Organs with [14C]CO2
    Authors:  Umesh P Yadav, Aswad S Khadilkar, Mearaj A Shaikh, Robert Turgeon and Brian G Ayre, date: 12/20/2017, view: 2301, Q&A: 0
    [Abstract] Phloem loading and transport of photoassimilate from photoautotrophic source leaves to heterotrophic sink organs are essential physiological processes that help the disparate organs of a plant function as a single, unified organism. We present three protocols we routinely use in combination with each other to assess (1) the relative rates of ...
    Assessing Rates of Long-distance Carbon Transport in Arabidopsis by Collecting Phloem Exudations into EDTA Solutions after Photosynthetic Labeling with [14C]CO2
    Authors:  Umesh P Yadav, Aswad S Khadilkar, Mearaj A Shaikh, Robert Turgeon and Brian G Ayre, date: 12/20/2017, view: 2422, Q&A: 0
    [Abstract] Phloem loading and transport of photoassimilate from photoautotrophic source leaves to heterotrophic sink organs are essential physiological processes that help the disparate organs of a plant function as a single, unified organism. We present three protocols we routinely use in combination with each other to assess (1) the relative rates of ...
    Rubisco Extraction and Purification from Diatoms
    [Abstract] This protocol describes a method to extract ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) from diatoms (Bacillariophyta) to determine catalytic performance. This protocol has been adapted from use in cyanobacteria and higher plants (Andrews, 1988; Whitney and Sharwood, 2007). First part (steps A1-A3) of the extraction provides ...
    Experimental Design to Determine Drought Stress Response and Early Leaf Senescence in Barley (Hordeum vulgare L.)
    Authors:  Gwendolin Wehner, Christiane Balko and Frank Ordon, date: 03/05/2016, view: 8367, Q&A: 0
    [Abstract] Premature leaf senescence induced by drought stress is a main factor for yield losses in barley. Research in drought stress tolerance has become more important as due to climate change the number of drought periods will increase and tolerance to drought stress has become a goal of high interest in barley breeding. However, reliable screening for ...
    Observation of Chloroplast Movement in Vallisneria
    Authors:  Yuuki Sakai and Shingo Takagi, date: 11/05/2015, view: 5075, Q&A: 0
    [Abstract] Chloroplasts accumulate to weak light and escape from strong light. These light-induced responses have been known from the 19th century (Böhm, 1856). Up to now, many scientists have developed different methods to investigate these dynamic phenomena in a variety of plant species including the model plant Arabidopsis thaliana, a terrestrial ...
    Chlorophyll Fluorescence Measurements in Arabidopsis Wild-type and Photosystem II Mutant Leaves
    Authors:  Iris Steinberger, Felix Egidi and Anja Schneider, date: 07/20/2015, view: 6122, Q&A: 0
    [Abstract] Chlorophyll fluorescence measurement is a widely used technique to determine photosynthetic performance. Light energy absorbed by a chlorophyll molecule can be dissipated by driving photochemical energy conversion, as heat in non-photochemical quenching processes, or it is re-emitted as fluorescence. The loss of light energy as chlorophyll ...
    Chlorophyll Fluorescence Measurements in Arabidopsis Plants Using a Pulse-amplitude-modulated (PAM) Fluorometer
    Authors:  Reiko Motohashi and Fumiyoshi Myouga, date: 05/05/2015, view: 10631, Q&A: 0
    [Abstract] In this protocol, to analyze PSII activity in photosynthesis, we measure the Fv/Fm (Fv=Fm ± Fo) value (Fo and Fm are the minimum and maximum values of chlorophyll fluorescence of dark-adapted leaves, respectively). Fv/Fm is a reliable marker of photo- inhibition (Krause et al., 1988). Chlorophyll fluorescence in leaves was measured at ...



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