Microbiology

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    Protocols in Current Issue
    Quantification of Trypanosoma cruzi in Tissue and Trypanosoma cruzi Killing Assay
    Authors:  Hisako Kayama, Shoko Kitada and Kiyoshi Takeda, date: 11/20/2017, view: 4758, Q&A: 0
    [Abstract] Infection with Trypanosoma cruzi causes Chagas disease. The methods provided here allow for the quantification of T. cruzi in the liver, heart, and blood of intraperitoneally-infected mice and analysis of the killing activity of the cells infected with T. cruzi in vitro.
    Gentamicin Protection Assay to Determine Bacterial Survival within Macrophages
    Authors:  Sargurunathan Subashchandrabose and Harry L.T. Mobley, date: 09/20/2014, view: 17968, Q&A: 0
    [Abstract] Macrophages are key cells involved in orchestrating host defense against infections. Here, we describe the protocol for a bacterial killing assay in macrophages that can be adapted to any bacterial pathogen. Using this assay, we analyzed the survival of wild-type and mutant strains of Escherichia coli (E. coli) within RAW 264.7 ...
    Isolation of Neutralizing Antibody
    Authors:  Mayo Yasugi and Kazuyoshi Ikuta, date: 12/20/2013, view: 7010, Q&A: 0
    [Abstract] Use of monoclonal antibodies (MAbs) is an established laboratory strategy for characterization of specific pathogens and their antigenicity. Especially, Human MAbs (HuMAbs) with neutralizing activity against specific virus could have potential therapeutic application, and provide significant information on human epitopes that could be important ...
    H2O2 Kill Assays of Planktonic Stationary Phase Bacteria
    Authors:  Malika Khakimova and Dao Nguyen, date: 11/05/2013, view: 7840, Q&A: 0
    [Abstract] Stationary phase bacteria are highly tolerant to hydrogen peroxide. This protocol was developed to test the susceptibility to hydrogen peroxide killing in different Pseudomonas aeruginosa strains. This assay provides a reliable way to measure killing of stationary phase bacterial cells to hydrogen peroxide and can be adapted to test other ...



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