Plant Science


    Protocols in Current Issue
    Simple Spectroscopic Determination of Nitrate, Nitrite, and Ammonium in Arabidopsis thaliana
    Authors:  Takushi Hachiya and Yuki Okamoto, date: 05/20/2017, view: 16774, Q&A: 2
    [Abstract] Plants use nitrate, nitrite, and ammonium as inorganic nitrogen (N) sources. These N compounds are included in plant tissues at various concentrations depending on the balance between their uptake and assimilation. Thus, the contents of nitrate, nitrite, and ammonium are physiological indicators of plant N economy. Here, we describe a protocol for ...
    In vitro Colorimetric Method to Measure Plant Glutamate Dehydrogenase Enzyme Activity
    Authors:  Asier Sarasketa, Izargi Vega-Mas and Daniel Marino, date: 08/20/2015, view: 10046, Q&A: 1
    [Abstract] Glutamate dehydrogenase (GDH) is an NAD(H) dependent enzyme that catalyzes, in vitro, the reversible amination of glutamate. Here we describe how to determine spectrophotometrically GDH activity monitoring NADH evolution. This protocol is described here for Arabidopsis thaliana (A. thaliana) although it is also valid for ...
    High-throughput Quantification of Ammonium Content in Arabidopsis
    Authors:  Izargi Vega-Mas, Asier Sarasketa and Daniel Marino, date: 08/20/2015, view: 9409, Q&A: 0
    [Abstract] This protocol is a simple colorimetric assay for internal ammonium quantification in aqueous extracts from plant tissues. The method is based on the phenol hypochlorite assay (Berthelot reaction):

    NH4+ + hypochlorite + OH- + phenol → indophenol

    The oxidation of indophenol caused by phenol ...
    Quantitative Evaluation of Competitive Nodulation among Different Sinorhizobium Strains
    Authors:  Chan Lan Chun, Matthew S. Nelson and Michael J. Sadowksy, date: 08/05/2015, view: 6456, Q&A: 0
    [Abstract] Legumes play a vital role in global food supply because they are uniquely capable of fixing atmospheric nitrogen (N) through symbioses with root and stem nodule bacteria, collectively called the rhizobia. These commonly include bacteria in the genera Rhizobium, Mesorhizobium, Sinorhizobium (Ensifer), and Bradyrhizobium ...
    Measurement of Net High-affinity Urea Uptake in Maize Plants
    Authors:  Laura Zanin, Nicola Tomasi and Roberto Pinton, date: 06/05/2015, view: 7698, Q&A: 0
    [Abstract] Despite its extensive use as a nitrogen fertilizer, the role of urea as a directly accessible nitrogen source for crop plants is still poorly understood. So far, the physiological and molecular aspects of urea acquisition have been investigated only in a few plant species highlighting the importance of a urea transporter in roots, DUR3 (Kojima et ...
    Determination of Nitrate Uptake and Accumulation Using 15N in Rice Seedlings
    Authors:  Zhong Tang and Guohua Xu, date: 08/20/2013, view: 7390, Q&A: 0
    [Abstract] Nitrogen-15 is a rare stable isotope of nitrogen. This isotope is often used in agricultural research. For example, Nitrogen-15 is used to trace mineral nitrogen compounds and translocate the nitrogen molecule in plants. This protocol is used to determine nitrate uptake and accumulation in rice seedlings by using Nitrogen-15.

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