Microbiology

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    Protocols in Current Issue
    Long-term in vitro Culture of Cryptosporidium parvum
    Authors:  Nigel Yarlett and Mary Morada, date: 08/05/2018, view: 1598, Q&A: 0
    [Abstract] Continuous in vitro growth of Cryptosporidium parvum has proved difficult and conventional in vitro culture techniques result in short-term (2-5 days) growth of the parasite resulting in thin-walled oocysts that fail to propagate using in vitro cultures, and do not produce an active infection using ...
    Alphavirus Purification Using Low-speed Spin Centrifugation
    Authors:  Vamseedhar Rayaprolu, Jolene Ramsey, Joseph Che-Yen Wang and Suchetana Mukhopadhyay, date: 03/20/2018, view: 2759, Q&A: 2
    [Abstract] Chemical and sedimentation procedures are used to purify virus particles. While these approaches are successful for wild-type viruses, they are often not feasible for purifying mutant viruses with assembly defects. We combined two published methods (Atasheva et al., 2013; Moller-Tank et al., 2013), to generate a protocol that ...
    Isolation of Commensal Escherichia coli Strains from Feces of Healthy Laboratory Mice or Rats
    Authors:  Tingting Ju and Benjamin P. Willing, date: 03/20/2018, view: 2622, Q&A: 0
    [Abstract] The colonization abundance of commensal E. coli in the gastrointestinal tract of healthy laboratory mice and rats ranges from 104 to 106 CFU/g feces. Although very well characterized, the family that E. coli belongs to has a very homogeneous 16S rRNA gene sequence, making the identification from 16S rRNA ...
    Isolation and Purification of Viruses Infecting Cyanobacteria Using a Liquid Bioassay Approach
    Authors:  Caroline Chénard and Amy M Chan, date: 01/20/2018, view: 2772, Q&A: 0
    [Abstract] The following protocol describes the isolation and purification of viruses infecting cyanobacteria using a liquid bioassay approach. Viruses infecting cyanobacteria are also known as cyanophages. This protocol was written specifically for the isolation of cyanophages infecting freshwater cyanobacteria particularly, cyanobacteria that cannot be ...
    A Bioreactor Method to Generate High-titer, Genetically Stable, Clinical-isolate Human Cytomegalovirus
    [Abstract] Human cytomegalovirus (HCMV) infection is a major cause of morbidity and mortality in transplant patients and a leading cause of congenital birth defects (Saint Louis, 2016). Vaccination and therapeutic studies often require scalable cell culture production of wild type virus, represented by clinical isolates. Obtaining sufficient stocks of ...
    Induction, Isolation and Counting of Akinetes in Aphanizomenon ovalisporum
    Authors:  Assaf Sukenik, Ruth N. Kaplan-Levi, Yehudit Viner-Mozzini, Achsa Lupu and Dotan Sela, date: 05/20/2016, view: 4296, Q&A: 0
    [Abstract] Akinetes are spore-like resting (dormant) cells formed by strains of filamentous cyanobacteria for surviving long periods of unfavorable conditions. During deprivation for potassium, vegetative photosynthetic cells along the filaments of the cyanobacterium Aphanizomenon ovalisporum (A. ovalisporum) (strain ILC-164) differentiate ...
    Establishing a Biofilm Co-culture of Pseudomonas and Aspergillus for Metabolite Extraction
    Authors:  He Zheng, Nancy P. Keller and Yun Wang, date: 12/05/2015, view: 6587, Q&A: 0
    [Abstract] Filamentous fungi and bacteria form mixed-species biofilms in nature and diverse clinical contexts (Frey-Klett et al., 2011; Peleg et al., 2010). The interactions between fungi and bacteria, often mediated by secreted metabolites, have important ramifications for the biology of the interacting partners (Frey-Klett et al. ...
    Isolation of Persister Cells from Biofilm and Planktonic Populations of Pseudomonas aeruginosa
    Author:  Cláudia N. H. Marques, date: 09/20/2015, view: 6833, Q&A: 0
    [Abstract] Persister cells are a stochastically produced sub-population of non-growing bacterial cells. Recently these cells have been more widely studied due to the recognition that they are tolerant to antimicrobials and thus, play a major role in the resilience of bacterial populations to antimicrobials, particularly in chronic biofilm infections. The ...
    Isolation of Rhizosphere Bacterial Communities from Soil
    Authors:  Laura J. White, Volker S. Brözel and Senthil Subramanian, date: 08/20/2015, view: 13960, Q&A: 0
    [Abstract] Rhizosphere bacterial communities have become a major focal point of research in recent years, especially regarding how they affect plants and vice versa (Philippot et al., 2013). Changes in microbial density and diversity within the rhizosphere occur in a spatial temporal manner. The soil zone closest to the plant roots has the most ...
    Quantitative Evaluation of Competitive Nodulation among Different Sinorhizobium Strains
    Authors:  Chan Lan Chun, Matthew S. Nelson and Michael J. Sadowksy, date: 08/05/2015, view: 4365, Q&A: 0
    [Abstract] Legumes play a vital role in global food supply because they are uniquely capable of fixing atmospheric nitrogen (N) through symbioses with root and stem nodule bacteria, collectively called the rhizobia. These commonly include bacteria in the genera Rhizobium, Mesorhizobium, Sinorhizobium (Ensifer), and Bradyrhizobium ...



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