Plant Science


    Protocols in Current Issue
    Polyamine and Paraquat Transport Assays in Arabidopsis Seedling and Callus
    Authors:  Haoxi Chai, Yun Shen and Huazhong Shi, date: 08/05/2017, view: 937, Q&A: 0
    [Abstract] Polyamines (PAs) are polycationic compounds found in all living organisms and play crucial roles in growth and survival. We here show the ‘Polyamine and paraquat (PQ) transport assay’ protocol, which can be used to examine the uptake activity of PA/PQ transporters. We have used this protocol to demonstrate that PUT3 in Arabidopsis is a ...
    Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints
    Authors:  Telma E. Scarpeci, María I. Zanor and Estela M. Valle, date: 06/20/2017, view: 1509, Q&A: 0
    [Abstract] Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With ...
    Measurement of Stomatal Conductance in Rice
    Authors:  Yin Wang and Toshinori Kinoshita, date: 04/20/2017, view: 1936, Q&A: 0
    [Abstract] Stomatal conductance, the reciprocal of stomatal resistance, represents the gas exchange ability of stomata. Generally, the stomatal conductance is higher when stomata open wider, and vice versa. In this protocol, we describe how to measure stomatal conductance in rice using Li-6400 (Licor, USA).
    Simple Methods for Screening and Statistical Analysis of Leaf Epidermal Cells in Dicotyledonous Plants
    Authors:  Jian-Jun Tao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2016, view: 2722, Q&A: 0
    [Abstract] Leaf epidermal cell size and number are positively correlated to leaf area. Stomata are specialized epidermal cells vital for gas exchange and water transpiration. So, observation and statistical analysis of the leaf epidermal cells are valuable for the study of leaf development and response to environmental stimulus. The classical method is using ...
    Measuring Auxin Transport Capacity in Seedling Roots of Medicago truncatula
    Authors:  Jason Liang Pin Ng and Ulrike Mathesius, date: 06/20/2016, view: 2871, Q&A: 0
    [Abstract] Measurement of auxin transport capacity provides quantitative data on the physiological machinery involved in auxin transport within plants. This technique is easy to perform and gives quick results. Radiolabelled auxin (indole-3-acetic-acid) is fed into the roots of Medicago truncatula via an agar block. The resulting radioactivity from ...
    Quantifying Auxin Metabolites in Young Root Tissue of Medicago truncatula by Liquid Chromatography Electrospray-ionisation Quadrupole Time-of-flight (LC-ESI-QTOF) Tandem Mass Spectrometry
    Authors:  Jason Liang Pin Ng, Thy T. Truong, Charles H. Hocart and Ulrike Mathesius, date: 06/20/2016, view: 2272, Q&A: 0
    [Abstract] Auxins represent a major group of phytohormones controlling plant development. The spatio-temporal regulation of auxin gradients is essential for the initiation, growth and correct development of plant organs. Because auxins and their metabolites occur at trace levels in plant tissue, experiments requiring identification plus their selective and ...
    Sample Preparation for X-ray Micro-computed Tomography of Woody Plant Material and Associated Xylem Visualisation Techniques
    Author:  Nicholas J. B. Brereton, date: 03/20/2016, view: 2781, Q&A: 0
    [Abstract] Variation in the tissue structure of short rotation coppice (SRC) willow is a principle factor driving differences in lignocellulosic sugar yield yet much of the physiology and development of this tissue is unknown. Traditional sectioning can be both difficult and destructive in woody tissue; however, technology such as three dimensional X-ray ...
    Cotyledon Wounding of Arabidopsis Seedlings
    Authors:  Debora Gasperini, Ivan F. Acosta and Edward E. Farmer, date: 01/20/2016, view: 3515, Q&A: 0
    [Abstract] Damage to plant organs through both biotic and abiotic injury is very common in nature. Arabidopsis thaliana 5-day-old (5-do) seedlings represent an excellent system in which to study plant responses to mechanical wounding, both at the site of the damage and in distal unharmed tissues. Seedlings of wild type, transgenic or mutant lines ...
    GC-MS-Based Analysis of Chloroform Extracted Suberin-Associated Root Waxes from Arabidopsis and Other Plant Species
    Authors:  Dylan K. Kosma, Isabel Molina and Owen Rowland, date: 12/20/2015, view: 3255, Q&A: 0
    [Abstract] The periderm and exodermis of taproots and tuberous taproots contain an extracellular lipid polymer, suberin, deposited in their cell walls. This polymer is intractable in organic solvents, and is co-deposited with chloroform-extractable waxes. These suberin-associated root waxes are typically composed of alkanes, primary alcohols, fatty acids, ...
    Chlorophyll Content Assay to Quantify the Level of Necrosis Induced by Different R Gene/Elicitor Combinations after Transient Expression
    Authors:  C. Jake Harris and David C. Baulcombe, date: 12/05/2015, view: 2365, Q&A: 0
    [Abstract] This assay can be used to rapidly and accurately quantify levels of leaf necrosis induced after transient expression of R genes and elicitor combinations (Harris et al., 2013). It is based on the inverse correlation between level of necrosis and chlorophyll content in leaf tissue. It is adapted from the calculations described by (Strain ...