Giant Mimiviridae CsCl Purification Protocol
Authors: Lionel Bertaux,
Audrey Lartigue and
Sandra Jeudy,
date: 11/20/2020,
view: 538,
Q&A: 0
[Abstract] While different giant viruses’ purification protocols are available, they are not fully described and they use sucrose gradient that does not reach an equilibrium. Here, we report a protocol for the purification of members of the Mimiviridae family virions resulting from Acanthamoeaba castellanii infections. Viruses are ...
Generation and in Planta Functional Analysis of Potato Virus Y mutants
Authors: Katja Stare,
Anna Coll,
Ion Gutiérrez-Aguirre,
Magda Tušek Žnidarič,
Maja Ravnikar,
Andreja Kežar,
Luka Kavčič,
Marjetka Podobnik and
Kristina Gruden,
date: 07/20/2020,
view: 835,
Q&A: 0
[Abstract] Potato virus Y (PVY), the type member of the genus Potyvirus (family Potyviridae), is the most widespread virus affecting potato and is included in the top five most economically detrimental plant viruses. Recently, the structure of the PVY virion has been determined by cryo-electron microscopy, which has opened the doors to ...
Intestinal Enteroid Culture for Human Astroviruses
[Abstract] Human astroviruses (HAstV) are non-enveloped, positive-sense single stranded RNA viruses that typically cause gastroenteritis in children, the elderly and among immunocompromised individuals. Some HAstV species have also been implicated in neurological diseases. It is important to study these viruses to understand the pathogenesis and develop ...
Purification of Rice Stripe Virus
Authors: Gang Lu,
Min Yao,
Yijun Zhou and
Xiaorong Tao,
date: 03/20/2020,
view: 1158,
Q&A: 0
[Abstract] Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice tissues. RSV virions with high infectivity were first precipitated ...
Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves
[Abstract] Given the endemic seroprevalence of herpes simplex viruses (HSV), its associated human diseases, and the emergence of acyclovir-resistant strains, there is a continuous need for better antiviral therapies. Towards this aim, identifying mechanistic details of how HSV-1 manipulates infected cells, how it modulates the immune responses, and how it ...