Stem Cell

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    Protocols in Current Issue
    Cell Microencapsulation and Cryopreservation with Low Molecular Weight Hyaluronan and Dimethyl Sulfoxide
    Authors:  H. Gurruchaga, L. Saenz del Burgo, G. Orive, R. M. Hernandez, J. Ciriza and J. L. Pedraz, date: 02/20/2019, view: 651, Q&A: 0
    [Abstract] Cryopreservation is commonly used for the storage of cells, tissues, organs or 3D cell-based products using ultra-low temperatures, which involves the immersion in liquid nitrogen for their long-term preservation. The cryopreservation of several microencapsulated cells is usually performed by the slow freezing with the dimethyl sulfoxide (DMSO) as ...
    Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue
    Authors:  Amit Sharma, Darilang Mawrie, Damarais Magdalene and Bithiah Grace Jaganathan, date: 02/20/2019, view: 664, Q&A: 0
    [Abstract] Mesenchymal stem cells (MSCs) have attracted significant attention as potential therapeutic cells to treat various diseases ranging from tissue injuries, graft versus host disease, degenerative diseases and cancer. Since the initial discovery of MSCs in the bone marrow cells, MSCs have been successfully isolated from various adult and neo-natal ...
    One-step Derivation of Functional Mesenchymal Stem Cells from Human Pluripotent Stem Cells
    Authors:  Yan Zhou, JinQi Liao, ChongZhou Fang, CuiPing Mo, GuangQian Zhou and Yonglun Luo, date: 11/20/2018, view: 1161, Q&A: 0
    [Abstract] Mesenchymal stem cells (MSCs) are invaluable cell sources for understanding stem cell biology and potential application in tissue engineering and regenerative medicine. The current issues of MSCs that demand to be further addressed are limited donors, tissue sources and limited capacity of ex vivo expansion. Here, we describe a simple and ...
    Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates
    [Abstract] The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological environment than classical 2D culture and has been used to enhance the MSC secretome or extend cell survival after transplantation. Here we describe a simple and affordable method to generate 3D spheroids of hMSCs by seeding them at high density in a low-binding ...
    Measurement of Mesenchymal Stem Cells Attachment to Endothelial Cells
    Authors:  Shan Wang, Chris D. Madsen and Yaojiong Wu, date: 03/20/2018, view: 2273, Q&A: 1
    [Abstract] Mesenchymal stem cells (MSCs) have shown profound therapeutic potential in tissue repair and regeneration. However, recent studies indicate that MSCs are largely entrapped in lungs after intravenous delivery and die shortly. The underlying mechanisms have been poorly understood. We have provided evidence to show that excess expression and ...
    Isolation and Establishment of Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord
    Authors:  Umesh Goyal, Chitra Jaiswal and Malancha Ta, date: 02/20/2018, view: 5449, Q&A: 4
    [Abstract] Mesenchymal stem cells (MSCs) are currently considered as ‘medicinal signaling cells’ and a promising resource in regard to cell-based regenerative therapy. Umbilical cord is a human term perinatal tissue which is easily attainable, and a promising source of stem cells with no associated ethical concerns. MSCs have been isolated from different ...
    Preparation of Amyloid Fibril Networks
    Authors:  Mirren Charnley , Jay Gilbert, Owen G. Jones and Nicholas P. Reynolds, date: 02/20/2018, view: 2449, Q&A: 0
    [Abstract] Networks of amyloid nanofibrils fabricated from common globular proteins such as lysozyme and β-lactoglobulin have material properties that mimic the extracellular microenvironment of many cell types. Cells cultured on such amyloid fibril networks show improved attachment, spreading and in the case of mesenchymal stem cells improved ...
    Human Endometrial Stem Cell Isolation from Endometrium and Menstrual Blood
    Authors:  Shima Tavakol, Freshteh Azedi, Elham Hoveizi, Jafar Ai and Mohammad Taghi Joghataei, date: 01/20/2018, view: 3111, Q&A: 0
    [Abstract] Human endometrial stem cell/stromal cells (hEnSCs) are isolated from endometrium or menstrual blood and are recognized as a valuable cell type in tissue engineering and cell therapy. Furthermore, hEnSCs, which have CD90 (a mesenchymal marker), CD105 (endoglin), CD44, CD146 (endometrial stem cell markers) and lack CD31 (Endothelial marker), CD34 ...
    A Co-culture Assay to Determine Efficacy of TNF-α Suppression by Biomechanically Induced Human Bone Marrow Mesenchymal Stem Cells
    Authors:  Miguel F. Diaz, Siobahn M. Evans, Scott D. Olson, Charles S. Cox Jr and Pamela L. Wenzel, date: 08/20/2017, view: 3545, Q&A: 0
    [Abstract] The beneficial effects of mesenchymal stem cell (MSC)-based cellular therapies are believed to be mediated primarily by the ability of MSCs to suppress inflammation associated with chronic or acute injury, infection, autoimmunity, and graft-versus-host disease. To specifically address the effects of frictional force caused by blood flow, or wall ...
    Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue
    Authors:  Natalia V. Andreeva, Alexandra A. Dalina and Alexander V. Belyavsky, date: 08/20/2017, view: 4951, Q&A: 1
    [Abstract] Mesenchymal stem cells (MSCs) are currently intensively studied due to significant promise which they represent for successful implementations of future cell therapy clinical protocols. This in turn emphasizes importance of careful preclinical studies of MSC effects in various murine disease models. The appropriate cell preparations with ...



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