Stem Cell

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    Protocols in Current Issue
    Investigating Neural Stem Cell and Glioma Stem Cell Self-renewal Potential Using Extreme Limiting Dilution Analysis (ELDA)
    Authors:  Hong PT Nguyen, Paul M Daniel, Gulay Filiz and Theo Mantamadiotis, date: 09/05/2018, view: 4253, Q&A: 0
    [Abstract] Glioma stem cells (GSC) grown as neurospheres exhibit similar characteristics to neural stem cells (NSC) grown as neurospheres, including the ability to self-renew and differentiate. GSCs are thought to play a role in cancer initiation and progression. Self-renewal potential of GSCs is thought to reflect many characteristics associated with ...
    Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells
    Authors:  Stefanie N. Bernas, Odette Leiter, Tara L. Walker and Gerd Kempermann, date: 11/05/2017, view: 10291, Q&A: 0
    [Abstract] There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be isolated and maintained in vitro, using two different culture systems to assess their potential regarding proliferation and differentiation ...
    Explant Methodology for Analyzing Neuroblast Migration
    Authors:  Kirsty J. Dixon, Alisa Turbic, Ann M. Turnley and Daniel J. Liebl, date: 05/05/2017, view: 6313, Q&A: 0
    [Abstract] The subventricular zone (SVZ) in the mammalian forebrain contains stem/progenitor cells that migrate through the rostral migratory stream (RMS) to the olfactory bulb throughout adulthood. SVZ-derived explant cultures provide a convenient method to assess factors regulating the intermediary stage of neural stem/progenitor cell migration. Here, we ...
    A Co-culture Model for Determining the Target Specificity of the de novo Generated Retinal Ganglion Cells
    Authors:  Pooja Teotia, Matthew J. Van Hook and Iqbal Ahmad, date: 04/05/2017, view: 5840, Q&A: 0
    [Abstract] In glaucoma, the output neurons of the retina, the retinal ganglion cells (RGCs), progressively degenerate, leading to irreversible blindness (Ahram et al., 2015). The ex vivo stem cell method to replace degenerated RGCs remains a potentially viable approach (Levin et al., 2004). However, the success of the approach ...
    Neurosphere Co-culture Assay
    Authors:  Fanny Ehret, Steffen Vogler and Gerd Kempermann, date: 08/05/2016, view: 9612, Q&A: 0
    [Abstract] The hippocampal niche is one of two areas in the brain where stem cells reside. In this neurogenic niche, stem cells can be found in close proximity to astrocytes and in contact with microvessels consisting of pericytes and endothelial cells. To study the regulatory interplay of this complex niche network in a simplified in vitro model, ...
    Neural Stem Cell Differentiation and Prion Infection
    Authors:  Yoshifumi Iwamaru, Takato Takenouchi and Hiroshi Kitani, date: 03/20/2014, view: 8862, Q&A: 0
    [Abstract] Prion diseases are transmissible, fatal, neurodegenerative diseases in human and animals. The molecular basis of neurodegeneration in prion diseases is largely unclear. Developing a cellular model capable of monitoring prion-induced cytotoxicity would be a promising approach for better understanding the prion pathogenesis. One candidate cellular ...
    Isolation and Culture of Neurospheres for the Study of Pathogenesis of Prion Disease
    Authors:  Yoshifumi Iwamaru, Takato Takenouchi and Hiroshi Kitani, date: 03/20/2014, view: 18207, Q&A: 0
    [Abstract] Neurosphere contains neural stem cells that are capable of self-renewal and multilineage differentiation including neurons, astrocytes, and oligodendrocytes (Gage, 2000). Cell culture model using differentiated neurosphere cultures are suggested to be a valuable tool for studying the pathogenesis of prion disease at the cellular level (Iwamaru et ...
    Primary Culture of SVZ-derived Progenitors Grown as Neurospheres
    Authors:  Julien Vernerey, Karine Magalon and Pascale Durbec, date: 08/20/2013, view: 9273, Q&A: 1
    [Abstract] SVZ-derived progenitors grown as neurospheres is a well-known model to study neural stem cell and progenitor functions such as proliferation, differentiation/self-renewal, and migration (Durbec and Rougon, 2001). This protocol is for preparing a culture of SVZ-derived progenitors from 8 early postnatal mouse brains (P0 to P3). One week after cell ...
    Neuronal Morphology Analysis
    Authors:  Désirée R. M. Seib and Ana Martin-Villalba, date: 08/05/2013, view: 9033, Q&A: 0
    [Abstract] This protocol describes how to visualize neuronal morphology and how to determine neuronal complexity of immature and mature hippocampal neurons in the mouse in vivo including tissue preparation, staining of brain sections and confocal cell analysis.
    In vivo Neurogenesis
    Authors:  Désirée R. M. Seib and Ana Martin-Villalba, date: 08/05/2013, view: 10772, Q&A: 0
    [Abstract] This protocol shows how to characterize the dynamics of hippocampal neurogenesis in the adult mouse by describing preparation of brain tissue, immunofluorescence of brain sections and confocal stereotactic cell counting.



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