Biochemistry

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    Protocols in Current Issue
    Quantification of Prostaglandin E2 Concentration in Interstitial Fluid from the Hypothalamic Region of Free-moving Mice
    Authors:  Yoshinobu Nakamura, Takatoshi Sakaguchi, Ikumi Tamai and Takeo Nakanishi, date: 08/05/2019, view: 1434, Q&A: 0
    [Abstract] Prostaglandin E2 (PGE2) is a well-established chemical mediator for the generation of the fever at the hypothalamus of the brain. PGE2 mediates fever generation via PGE receptor 3 (i.e., EP3) on neurons in the preoptic area. The role of PGE2 has been analyzed by measuring PGE2 ...
    Optimized Oxidative Stress Protocols for Low-microliter Volumes of Mammalian Plasma
    Author:  Evan A. Langille, date: 05/05/2019, view: 1516, Q&A: 0
    [Abstract] Small blood volumes commonly obtained from small mammals during field studies are only sufficient for a single biochemical assay. In this study, we used blood collected from a population of wild eastern chipmunks (Tamias striatus) and developed modified methods to improve analytical selectivity and sensitivity required for measuring ...
    I Plate-based Assay for Studying How Fungal Volatile Compounds (VCs) Affect Plant Growth and Development and the Identification of VCs via SPME-GC-MS
    Authors:  Wenzhao Wang, Ningxiao Li, Xingzhong Liu and Seogchan Kang, date: 02/20/2019, view: 2909, Q&A: 0
    [Abstract] Biogenic volatile compounds (VCs) mediate various types of crucial intra- and inter-species interactions in plants, animals, and microorganisms owing to their ability to travel through air, liquid, and porous soils. To study how VCs produced by Verticillium dahliae, a soilborne fungal pathogen, affect plant growth and development, we ...
    Extraction of Small Molecules from Fecal Samples and Testing of Their Activity on Microbial Physiology
    Authors:  Eduardo S. Alves, Rosana B. R. Ferreira and L. Caetano M. Antunes, date: 04/20/2018, view: 3686, Q&A: 0
    [Abstract] The human body is colonized by vast communities of microbes, collectively known as microbiota, or microbiome. Although microbes colonize every surface of our bodies that is exposed to the external environment, the biggest collection of microbes colonizing humans and other mammals can be found in the gastrointestinal tract. Given the fact that the ...



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