Neuroscience

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    Protocols in Current Issue
    Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina
    Authors:  Sowmya Bekshe Lokappa, M. Carter Cornwall and Jeannie Chen, date: 07/20/2019, view: 1264, Q&A: 0
    [Abstract] Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its ability to activate the visual G-protein transducin. Binding of light-activated, ...
    Embryonic Intravitreous Injection in Mouse
    Authors:  Liyuan Cui, Yupu Diao and Jiayi Zhang, date: 07/20/2018, view: 1584, Q&A: 0
    [Abstract] Axons of retinal ganglion cells (RGCs) relay visual information from the retina to lateral geniculate nucleus (LGN) and superior colliculus (SC), which are two major image-forming visual nuclei. Wiring of these retinal projections completes before vision begins. However, there are few studies on retinal axons at embryonic stage due to technical ...
    Registration and Alignment Between in vivo Functional and Cytoarchitectonic Maps of Mouse Visual Cortex
    Authors:  Jun Zhuang, Quanxin Wang, Marc Takeno and Jack Waters, date: 02/20/2018, view: 2197, Q&A: 0
    [Abstract] This protocol describes a method for registration of in vivo cortical retinotopic map with cytochrome c oxidase (CO) labeled architectonic maps of the same mouse brain through the alignment of vascular fiducials. By recording surface blood vessel pattern and sequential alignment at each step, this method overcomes the challenge imposed by ...



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