Cell Biology

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    Protocols in Current Issue
    Fractionation of Ovarian Follicles and in vitro Oocyte Maturation and Ovulation Assay of Ciona intestinalis Type A
    Authors:  Shin Matsubara, Akira Shiraishi, Tomohiro Osugi, Tsuyoshi Kawada and Honoo Satake, date: 04/05/2020, view: 973, Q&A: 0
    [Abstract] Ascidians are the closest living relatives of vertebrates (Delsuc et al., 2006; Satoh et al., 2014) and are important for the evolutionary study of the ovarian follicle development including oocyte maturation and ovulation. However, neither the endogenous factors nor the molecular mechanisms underlying the oocyte maturation and ...
    Mouse Embryonic Tooth Germ Dissection and Ex vivo Culture Protocol
    Authors:  Xue Han, Keigo Yoshizaki, Tian Tian, Kanako Miyazaki, Ichiro Takahashi and Satoshi Fukumoto, date: 02/05/2020, view: 1408, Q&A: 1
    [Abstract] A tooth germ ex vivo organ culture allows visualization of its development in different stages, thus enabling investigation of the molecular mechanisms of regulatory factors. Tooth germs can be rapidly dissected from E13 mouse embryos and placed on cell culture inserts for observation of subsequent tooth germ development in a ...
    Sebinger Culture: A System Optimized for Morphological Maturation and Imaging of Cultured Mouse Metanephric Primordia
    Authors:  Mona Elhendawi and Jamie A. Davies, date: 02/20/2018, view: 3304, Q&A: 0
    [Abstract] Here, we present a detailed protocol on setting up embryonic renal organ cultures using a culture method that we have optimised for anatomical maturation and imaging. Our culture method places kidney rudiments on glass in a thin film of medium, which results in very flat cultures with all tubules in the same image plane. For reasons not yet ...



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