Cellular mechanisms —Nervous system disorders —Neuroscience

Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein

Authors: Sayan Dutta, Ben G. Watson, Seema Mattoo and Jean-Christophe Rochet, date: 07/20/2020, view: 271, Q&A: 0

[Abstract] Lipid membranes are involved in regulating biochemical and biological processes and in modulating the selective permeability of cells, organelles, and vesicles. Membrane composition, charge, curvature, and fluidity all have concerted effects on cellular signaling and homeostasis. The ability to prepare artificial lipid assemblies that mimic ...

An ex vivo Approach to Assess Mitochondrial ROS by Flow Cytometry in AAV-tagged Astrocytes in Adult Mice

Authors: Carlos Vicente-Gutierrez and Juan P. Bolaños, date: 03/20/2020, view: 1017, Q&A: 0

[Abstract] Mitochondrial reactive oxygen species (mROS) are naturally produced signalling molecules extremely relevant for understanding both health- and disease-associated biological processes. The study of mROS in the brain is currently underway to decipher their physiopathological roles and contributions in neurological diseases. Recent advances in this ...

Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease

Authors: Siddhartha Mondragón-Rodríguez, Benito Ordaz, Erika Orta-Salazar, Sofia Díaz-Cintra, Fernando Peña-Ortega and George Perry, date: 02/20/2020, view: 927, Q&A: 0

[Abstract] Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of ...

Mitochondrial Respiratory Measurements in Patient-derived Fibroblasts

Authors: Prashant Mishra, Ting Zhang, Ming Guo and David Chan, date: 12/05/2019, view: 1339, Q&A: 0

[Abstract] Mitochondrial dysfunction is associated with a number of human diseases. As an example, we recently established in vivo Drosophila models of IBMPFD (Inclusion body myopathy, Paget disease, and frontotemporal dementia), and uncovered that human disease mutations of the p97/VCP (Valosin Containing Protein) gene behave as ...

Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification

Authors: Joseph McInnes, Lujia Zhou and Patrik Verstreken, date: 11/20/2018, view: 3633, Q&A: 0

[Abstract] Dysfunction of the microtubule-associated protein Tau (encoded by the MAPT gene) has been implicated in more than twenty neurodegenerative diseases, including Alzheimer’s. As such, the physiological and disease-relevant functions of Tau have garnered great interest in the research community. One barrier hampering investigations into the ...

Ex vivo Whole-cell Recordings in Adult Drosophila Brain

Authors: Alexa J. Roemmich, Soleil S. Schutte and Diane K. O’Dowd, date: 07/20/2018, view: 3732, Q&A: 0

[Abstract] Cost-effective and efficient, the fruit fly (Drosophila melanogaster) has been used to make many key discoveries in the field of neuroscience and to model a number of neurological disorders. Great strides in understanding have been made using sophisticated molecular genetic tools and behavioral assays. Functional analysis of neural ...

Intracellular and Mitochondrial Reactive Oxygen Species Measurement in Primary Cultured Neurons

Authors: Seung Hyun Baek, Yoonsuk Cho, Jeongmi Lee, Bo Youn Choi, Yuri Choi, Jin Su Park, Harkkyun Kim, Jaehoon Sul, Eunae Kim, Jae Hyung Park and Dong-Gyu Jo, date: 06/05/2018, view: 5356, Q&A: 0

[Abstract] Reactive oxygen species (ROS) are chemically reactive oxygen containing molecules. ROS consist of radical oxygen species including superoxide anion (O₂^•−) and hydroxyl radical (•OH) and non-radical oxygen species such as hydrogen peroxide (H₂O₂), singlet oxygen (O₂). ROS are generated by ...

Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice

Authors: Carmelo Bellardita, Maite Marcantoni, Peter Löw and Ole Kiehn, date: 04/05/2018, view: 4091, Q&A: 0

[Abstract] Spinal cord injury (SCI) is characterized by multiple sensory/motor impairments that arise from different underlying neural mechanisms. Linking specific sensory/motor impairments to neural mechanism is limited by a lack of direct experimental access to these neural circuits. Here, we describe an experimental model which addresses this shortcoming. ...

Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons

Authors: Katarina Trajkovic, Hyunkyung Jeong and Dimitri Krainc, date: 01/05/2018, view: 4035, Q&A: 0

[Abstract] Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of mutant huntingtin (mHtt) secreted from Neuro2A cell line stably expressing mHtt and rat primary ...

Obtaining Multi-electrode Array Recordings from Human Induced Pluripotent Stem Cell–Derived Neurons

Authors: Xiaohong Xu, Carola I. Radulescu, Kagistia Hana Utami and Mahmoud A. Pouladi , date: 11/20/2017, view: 7403, Q&A: 0

[Abstract] Neuronal electrical properties are often aberrant in neurological disorders. Human induced pluripotent stem cells (hiPSCs)-derived neurons represent a useful platform for neurological disease modeling, drug discovery and toxicity screening in vitro. Multi-electrode array (MEA) systems offer a non-invasive and label-free platform to record ...

Neuroscience

Multiple Simultaneous Acute Stresses in Mice: Single or Repeated Induction

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