Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein
[Abstract] Lipid membranes are involved in regulating biochemical and biological processes and in modulating the selective permeability of cells, organelles, and vesicles. Membrane composition, charge, curvature, and fluidity all have concerted effects on cellular signaling and homeostasis. The ability to prepare artificial lipid assemblies that mimic ...
Ex vivo Whole-cell Recordings in Adult Drosophila Brain
[Abstract] Cost-effective and efficient, the fruit fly (Drosophila melanogaster) has been used to make many key discoveries in the field of neuroscience and to model a number of neurological disorders. Great strides in understanding have been made using sophisticated molecular genetic tools and behavioral assays. Functional analysis of neural ...
Intracellular and Mitochondrial Reactive Oxygen Species Measurement in Primary Cultured Neurons
Authors: Seung Hyun Baek,
Yoonsuk Cho,
Jeongmi Lee,
Bo Youn Choi,
Yuri Choi,
Jin Su Park,
Harkkyun Kim,
Jaehoon Sul,
Eunae Kim,
Jae Hyung Park and
Dong-Gyu Jo,
date: 06/05/2018,
view: 6366,
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[Abstract] Reactive oxygen species (ROS) are chemically reactive oxygen containing molecules. ROS consist of radical oxygen species including superoxide anion (O2•−) and hydroxyl radical (•OH) and non-radical oxygen species such as hydrogen peroxide (H2O2), singlet oxygen (O2). ROS are generated by ...
Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
[Abstract] Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of mutant huntingtin (mHtt) secreted from Neuro2A cell line stably expressing mHtt and rat primary ...