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VirScan: High-throughput Profiling of Antiviral Antibody Epitopes

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An Optimized Tat/Rev Induced Limiting Dilution Assay for the Characterization of HIV-1 Latent Reservoirs

Authors: Swarnima Mishra, Yuvrajsinh Gohil, Kavita Mehta, Anish D'silva, Afzal Amanullah, Deepak Selvam, Neelam Pargain, Narendra Nala, G. N. Sanjeeva and Udaykumar Ranga, date: 04/20/2022, view: 521, Q&A: 0

[Abstract]

The administration of antiretroviral therapy (ART) leads to a rapid reduction in plasma viral load in HIV-1 seropositive subjects. However, when ART is suspended, the virus rebounds due to the presence of a latent viral reservoir. Several techniques have been developed to characterize this latent viral reservoir. Of the various assay formats

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Suppression of Human Dendritic Cells by Regulatory T Cells

Authors: Qing Huang, Avery J. Lam, Dominic A. Boardman, Nicholas A. J. Dawson and Megan K. Levings, date: 11/05/2021, view: 1703, Q&A: 0

[Abstract]

Regulatory T cells (Tregs) suppress immune responses via a variety of mechanisms and can be used as a cellular therapy to induce tolerance. The function of Tregs is commonly assessed in vitro using assays that measure suppression of effector T cell proliferation and/or cytokine production. However, Tregs can also suppress the function of antigen

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In vitro Differentiation of Thymic T_reg Cell Progenitors to Mature Thymic T_reg Cells

Authors: David L. Owen and Michael A. Farrar, date: 08/20/2019, view: 3055, Q&A: 0

[Abstract] Thymic T_reg cell differentiation occurs via a two-step process. Step one generates T_reg cell progenitors (T_regP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated by activation of STAT5 via IL-2, or IL-15, leading to mature T_reg cells capable of ...

Ultrasound Guided Intra-thymic Injection to Track Recent Thymic Emigrants and Investigate T Cell Development

Authors: Haiguang Wang, David L. Owen, Lily J. Qian, Laura B. Chopp, Michael A. Farrar and Kristin A. Hogquist, date: 12/05/2018, view: 3736, Q&A: 0

[Abstract] To track recent thymic emigrants (RTEs) or study T cell development in the thymus, intra-thymic injection of a cellular tag or precursor cells for various T cell lineages is often desired. However, the traditional surgical approach to expose the thymus for intra-thymic injection is time-consuming and can cause a high level of pain and stress to ...

Generation of Busulfan Chimeric Mice for the Analysis of T Cell Population Dynamics

Authors: Thea Hogan, Andrew Yates and Benedict Seddon, date: 12/20/2017, view: 8831, Q&A: 0

[Abstract] This protocol was developed to generate chimeric mice in which T lymphocytes could be stratified by age on the basis of congenic marker expression. The conditioning drug busulfan is used to ablate host haematopoietic stem cells while leaving the peripheral immune system intact. Busulfan treatment is followed by bone marrow transplantation (BMT), ...

In vitro Homeostatic Proliferation of Human CD8 T Cells

Authors: Hossam A. Abdelsamed, Caitlin C. Zebley and Ben Youngblood, date: 11/20/2017, view: 9522, Q&A: 0

[Abstract] Long-lived T-cell–mediated immunity requires persistence of memory T cells in an antigen-free environment while also maintaining a heightened capacity to recall effector functions. Such antigen-independent homeostatic proliferation is mediated in part by the common gamma-chain cytokines IL-7 and IL-15. To further explore the mechanisms governing ...

Efficient Production of Functional Human NKT Cells from Induced Pluripotent Stem Cells − Reprogramming of Human Vα24⁺iNKT Cells

Authors: Daisuke Yamada, Tomonori Iyoda, Kanako Shimizu, Yusuke Sato, Haruhiko Koseki and Shin-ichiro Fujii, date: 05/20/2017, view: 10585, Q&A: 0

[Abstract] Antigen-specific T cell-derived induced pluripotent stem cells (iPSCs) have been shown to re-differentiate into functional T cells and thus provide a potential source of T cells that could be useful for cancer immunotherapy. Human Vα24⁺ invariant natural killer T (Vα24⁺iNKT) cells are subset of T cells that are characterized ...

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