Microscopy-based Methods for Rosetting Assay in Malaria Research
Authors: Wenn-Chyau Lee and
Laurent Rénia,
date: 07/05/2020,
view: 716,
Q&A: 0
[Abstract] In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune escape mechanism for the parasite. However, it has been associated with malaria pathogenesis, possibly ...
Testing Bone Formation Induction by Calvarial Injection Assay in vivo
[Abstract] Bone formation occurs during embryogenesis, skeletal growth and during the process of skeletal renewal throughout life. In the process of bone formation, osteoblasts lay down a collagen-containing matrix, termed osteoid, which is gradually hardened by incorporation of mineral crystals. Although osteoblasts can be induced to differentiate and to ...
Endocytosis Detection in Magnaporthe oryzae
Authors: Muxing Liu and
Zhengguang Zhang,
date: 08/05/2019,
view: 1533,
Q&A: 0
[Abstract] Endocytosis is an intracellular trafficking pathway that occurs in nutrient uptake, signal transduction and reconstruction of cell polarity and is conserved in eukaryotic cells. In fungi, endocytosis plays crucial roles in the physiology of hyphal growth and pathogenicity. vidence for endocytosis in filamentous fungi is detected by the ...
Visualizing Filamentous Actin in Chlamydomonas reinhardtii
Authors: Evan W. Craig and
Prachee Avasthi,
date: 06/20/2019,
view: 2441,
Q&A: 0
[Abstract] This protocol aims to visualize the filamentous actin network in Chlamydomonas reinhardtii. We improved fixed-cell labeling conditions using the F-actin probe, phalloidin. We created a Chlamydomonas-optimized protocol by halving the phalloidin incubation time, electing for optimal fixation conditions, and selecting for a healthy ...