Generation and in Planta Functional Analysis of Potato Virus Y mutants
Authors: Katja Stare,
Anna Coll,
Ion Gutiérrez-Aguirre,
Magda Tušek Žnidarič,
Maja Ravnikar,
Andreja Kežar,
Luka Kavčič,
Marjetka Podobnik and
Kristina Gruden,
date: 07/20/2020,
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Q&A: 0
[Abstract] Potato virus Y (PVY), the type member of the genus Potyvirus (family Potyviridae), is the most widespread virus affecting potato and is included in the top five most economically detrimental plant viruses. Recently, the structure of the PVY virion has been determined by cryo-electron microscopy, which has opened the doors to ...
Intestinal Enteroid Culture for Human Astroviruses
[Abstract] Human astroviruses (HAstV) are non-enveloped, positive-sense single stranded RNA viruses that typically cause gastroenteritis in children, the elderly and among immunocompromised individuals. Some HAstV species have also been implicated in neurological diseases. It is important to study these viruses to understand the pathogenesis and develop ...
Microscopy-based Methods for Rosetting Assay in Malaria Research
Authors: Wenn-Chyau Lee and
Laurent Rénia,
date: 07/05/2020,
view: 687,
Q&A: 0
[Abstract] In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune escape mechanism for the parasite. However, it has been associated with malaria pathogenesis, possibly ...
Differential Fractionation of Erythrocytes Infected by Plasmodium berghei
[Abstract] The study of host/pathogen interactions at the cellular level during Plasmodium intra-erythrocytic cycle requires differential extraction techniques aiming to analyze the different compartments of the infected cell. Various protocols have been proposed in the literature to study specific compartments and/or membranes in the infected ...