Cancer Biology

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    Protocols in Current Issue
    Modeling NOTCH1 driven T-cell Acute Lymphoblastic Leukemia in Mice
    Authors:  Agnieszka A. Wendorff and Adolfo A. Ferrando, date: 05/20/2020, view: 1022, Q&A: 0
    [Abstract] T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy that arises from transformation of T-cell primed hematopoietic progenitors. Although T-ALL is a heterogenous and molecularly complex disease, more than 65% of T-ALL patients carry activating mutations in the NOTCH1 gene. The majority of T-ALL–associated ...
    Isolation of Stem Cells, Endothelial Cells and Pericytes from Human Infantile Hemangioma
    Authors:  Lan Huang and Joyce Bischoff, date: 01/20/2020, view: 1062, Q&A: 0
    [Abstract] Infantile hemangioma (IH) is a vascular tumor noted for its excessive blood vessel formation during infancy, glucose-transporter-1 (GLUT1)-positive staining of the blood vessels, and its slow spontaneous involution over several years in early childhood. For most children, IH poses no serious threat because it will eventually involute, but a subset ...
    Investigating Neural Stem Cell and Glioma Stem Cell Self-renewal Potential Using Extreme Limiting Dilution Analysis (ELDA)
    Authors:  Hong PT Nguyen, Paul M Daniel, Gulay Filiz and Theo Mantamadiotis, date: 09/05/2018, view: 4059, Q&A: 0
    [Abstract] Glioma stem cells (GSC) grown as neurospheres exhibit similar characteristics to neural stem cells (NSC) grown as neurospheres, including the ability to self-renew and differentiate. GSCs are thought to play a role in cancer initiation and progression. Self-renewal potential of GSCs is thought to reflect many characteristics associated with ...
    3D Culture Protocol for Testing Gene Knockdown Efficiency and Cell Line Derivation
    Authors:  Jan Strnadel, Sang Myung Woo, Sunkyu Choi, Huawei Wang, Marian Grendar and Ken Fujimura, date: 06/05/2018, view: 5794, Q&A: 0
    [Abstract] Traditional 2D cell cultures with cells grown as monolayers on solid surface still represent the standard method in cancer research for drug testing. Cells grown in 2D cultures, however, lack relevant cell-matrix and cell-cell interactions and ignore the true three-dimensional anatomy of solid tumors. Cells cultured in 2D can also undergo ...
    Primary Cultures from Human GH-secreting or Clinically Non-functioning Pituitary Adenomas
    Authors:  Roberto Würth, Alessandra Pattarozzi, Federica Barbieri and Tullio Florio, date: 04/05/2018, view: 3731, Q&A: 0
    [Abstract] Pituitary adenomas are among the more frequent intracranial tumors usually treated with both surgical and pharmacological–based on somatostatin and dopamine agonists–approaches. Although mostly benign tumors, the occurrence of invasive behaviors is often detected resulting in poorer prognosis. The use of primary cultures from human pituitary ...
    FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand
    Authors:  Jessica Tome-Garcia, Fiona Doetsch and Nadejda M. Tsankova, date: 12/20/2017, view: 7503, Q&A: 0
    [Abstract] Direct isolation of human neural and glioma stem cells from fresh tissues permits their biological study without prior culture and may capture novel aspects of their molecular phenotype in their native state. Recently, we demonstrated the ability to prospectively isolate stem cell populations from fresh human germinal matrix and glioblastoma ...
    Myeloid Progenitor Transformation Assay
    Authors:  Hiroshi Okuda and Akihiko Yokoyama, date: 12/05/2017, view: 4461, Q&A: 0
    [Abstract] Numerous oncogenes have been identified to cause leukemia. For example, chromosomal translocation generates various fusion genes of the mixed-lineage leukemia (MLL) gene and a partner gene in leukemia, whose gene products transform primary myeloid progenitors into an immortalized state. To characterize the transforming ability of leukemic ...
    Isolation and Separation of Epithelial CD34+ Cancer Stem Cells from Tgfbr2-deficient Squamous Cell Carcinoma
    Authors:  Heather A. McCauley and Géraldine Guasch, date: 09/05/2017, view: 5378, Q&A: 0
    [Abstract] Most epithelial tumors have been shown to contain cancer stem cells that are potentially the driving force in tumor progression and metastasis (Kreso and Dick, 2014; Nassar and Blanpain, 2016). To study these cells in depth, cell isolation strategies relying on cell surface markers or fluorescent reporters are essential, and the isolation ...
    Teratoma Formation Assay for Assessing Pluripotency and Tumorigenicity of Pluripotent Stem Cells
    Authors:  Shingo Miyawaki, Yohei Okada, Hideyuki Okano and Kyoko Miura, date: 08/20/2017, view: 9717, Q&A: 0
    [Abstract] Pluripotent stem cells such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) form teratomas when transplanted into immunodeficient mice. As teratomas contain all three germ layers (endoderm, mesoderm, ectoderm), teratoma formation assay is widely used as an index of pluripotency (Evans and Kaufman, 1981; Hentze et al. ...
    Tumorigenicity Assay in Nude Mice
    Authors:  Feng Du, Xiaodi Zhao and Daiming Fan, date: 07/05/2017, view: 13945, Q&A: 0
    [Abstract] Tumorigenicity refers to the ability of cultured cells to develop viable tumors in immune-deficient animals. The goal of this protocol is to illustrate tumorigenicity assay by subcutaneous tumor-cell-transplantation in nude mice. Target cells are transplanted to 6-week-old nude mice subcutaneously and the tumor growth is monitored over a period of ...



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