Neuroscience

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    Protocols in Current Issue
    An Improved Method for Individual Tracking of Voluntary Wheel Running in Pair-housed Juvenile Mice
    Authors:  David A. Valientes, Anthony M. Raus and Autumn S. lvy, date: 07/05/2021, view: 660, Q&A: 0
    [Abstract]

    Rodent cages equipped with access to a voluntary running wheel are commonly used to study the effects of aerobic physical activity on physiology and behavior. Notable discoveries in exercise neurobiology, including the key role of brain-derived neurotrophic factor (BDNF) in neural plasticity and cognition, have been made using rodents housed with

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    Evaluating Baseline and Sensitised Heat Nociception in Adult Drosophila
    Authors:  Josephine N. Massingham, Olga Baron and G. Gregory Neely, date: 07/05/2021, view: 543, Q&A: 0
    [Abstract]

    Chronic pain is a complex disease that affects a large proportion of the population. With little to no effective treatments currently available for patients, this malady presents a large burden to society. Drosophila melanogaster has been previously used to describe conserved molecular components of nociception in larvae and adults. However, adult

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    Objective Quantitation of Focal Sweating Areas Using a Mouse Sweat-assay Model
    Authors:  Choongjin Ban and Dae-Hyuk Kwoen, date: 06/05/2021, view: 1026, Q&A: 0
    [Abstract]

    In vivo sweat quantitation assays are required for the development of drugs for the management of focal hyperhidrosis before clinical trials; however, in vivo assays, particularly mouse models, are rare. Even in sweat assays using mice, sweating is quantitated by manually counting the number of sweating spots, which can contribute to various

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    Single or Repeated Ablation of Mouse Olfactory Epithelium by Methimazole
    Authors:  Sofia Håglin, Staffan Bohm and Anna Berghard, date: 04/20/2021, view: 1896, Q&A: 0
    [Abstract]

    Odor-detecting olfactory sensory neurons residing in the nasal olfactory epithelium (OE) are the only neurons in direct contact with the external environment. As a result, these neurons are subjected to chemical, physical, and infectious insults, which may be the underlying reason why neurogenesis occurs in the OE of adult mammals. This feature

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    Low- and High-resolution Dynamic Analyses for Magnetic Resonance Spectroscopy Data
    Author:  Reuben Rideaux, date: 01/20/2021, view: 1340, Q&A: 0
    [Abstract]

    Magnetic resonance spectroscopy (MRS) can be used to measure in vivo concentrations of neurometabolites. This information can be used to identify neurotransmitter involvement in healthy (e.g., perceptual and cognitive processes) and unhealthy brain function (e.g., neurological and psychiatric illnesses). The standard approach for analyzing MRS

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    Headpost Surgery for in vivo Electrophysiological Recording in the Mouse Inferior Colliculus during Locomotion
    Authors:  Yoonsun Yang and Gunsoo Kim, date: 12/05/2020, view: 1042, Q&A: 0
    [Abstract]

    The inferior colliculus (IC) is a critical midbrain integration center for auditory and non-auditory information. Although much is known about the response properties of the IC neurons to auditory stimuli, how the IC neural circuits function during movement such as locomotion remains poorly understood. Mice offer a valuable model in this respect,

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    Probe-Seq: Method for RNA Sequencing of Specific Cell Types from Animal Tissue
    [Abstract] Most organs and tissues are composed of many types of cells. To characterize cellular state, various transcription profiling approaches are currently available, including whole-tissue bulk RNA sequencing, single cell RNA sequencing (scRNA-Seq), and cell type-specific RNA sequencing. What is missing in this repertoire is a simple, versatile method ...
    Long-term Devocalization of Zebra Finches
    Authors:  Yunbok Kim, Daisuke Mizuguchi and Satoshi Kojima, date: 09/20/2020, view: 1874, Q&A: 0
    [Abstract] Songbirds, such as the zebra finch, are a popular animal model for studying the neural basis of vocal and complex skill learning. Adult male zebra finches produce courtship song toward females (referred to as ‘directed song’) and recording and analyzing sounds of directed song along with underlying neural activity is important for investigating ...
    Sequential Reaching Task for the Study of Motor Skills in Monkeys
    Authors:  Machiko Ohbayashi and Nathalie Picard, date: 08/20/2020, view: 1280, Q&A: 0
    [Abstract] The ability to perform a sequence of movements is a key component of motor skills, such as typing or playing a musical instrument. How the brain binds elementary movements together into meaningful actions has been a topic of much interest. Here, we describe two sequential reaching tasks that we use to investigate the neural substrate of skilled ...
    A Reproducible Protocol to Measure the Critical Swimming Speed of Adult Zebrafish
    Authors:  Yuma Wakamatsu, Makoto Kashima and Hiromi Hirata, date: 08/20/2020, view: 2207, Q&A: 1
    [Abstract] The quantitative measurement of water flow-induced swimming of fish species using a swimmill is a powerful method to evaluate motor ability of individual fish. Zebrafish is a commonly used vertebrate that enables the study of morphological, physiological and behavioral characteristics associated with genes. We here established a reproducible ...



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