A Mouse Model of Postoperative Pain
Authors: Ashley M. Cowie and
Cheryl L. Stucky,
date: 01/20/2019,
view: 1317,
Q&A: 0
[Abstract] Postoperative pain is highly debilitating and hinders recovery. Opioids are the main pain medication used for acute postoperative pain. Given the devastating opioid addiction and overdose epidemic across the US, non-opioid pain therapeutics are desperately needed. In order to develop novel, non-opioid therapies for the treatment of postoperative ...
Artificial Inhalation Protocol in Adult Mice
Authors: Thomas P. Eiting and
Matt Wachowiak,
date: 09/20/2018,
view: 1055,
Q&A: 0
[Abstract] Research in the area of in vivo olfactory physiology benefits from having direct access to the nasal airways through which odorants can be presented. Ordinarily, the passage of odorants through the airways is controlled by respiratory rhythm. This fact makes it difficult to control the timing and strength of an olfactory stimulus, since ...
Embryonic Intravitreous Injection in Mouse
Authors: Liyuan Cui,
Yupu Diao and
Jiayi Zhang,
date: 07/20/2018,
view: 1218,
Q&A: 0
[Abstract] Axons of retinal ganglion cells (RGCs) relay visual information from the retina to lateral geniculate nucleus (LGN) and superior colliculus (SC), which are two major image-forming visual nuclei. Wiring of these retinal projections completes before vision begins. However, there are few studies on retinal axons at embryonic stage due to technical ...
A Fluorescent Dye Method Suitable for Visualization of One or More Rat Whiskers
[Abstract] Visualization and tracking of the facial whiskers is critical to many studies of rodent behavior. High-speed videography is the most robust methodology for characterizing whisker kinematics, but whisker visualization is challenging due to the low contrast of the whisker against its background. Recently, we showed that fluorescent dye(s) can be ...
Construction and Cloning of Minigenes for in vivo Analysis of Potential Splice Mutations
[Abstract] Disease-associated mutations influencing mRNA splicing are referred to as splice mutations. The majority of splice mutations are found on exon-intron boundaries defining canonical donor and acceptor splice sites. However, mutations in the coding region (exonic mutations) can also affect mRNA splicing. Exact knowledge of the disease mechanism of ...
Assaying Thermo-nociceptive Behavior in Drosophila Larvae
[Abstract] Thermo-nociception, the detection and behavioral response to noxious temperatures, is a highly conserved action to avoid injury and ensure survival. Basic molecular mechanisms of thermal responses have been elucidated in several model organisms and are of clinical relevance as thermal hypersensitivity (thermos-allodynia) is common in neuropathic ...
Assaying Mechanonociceptive Behavior in Drosophila Larvae
[Abstract] Drosophila melanogaster larvae have been extensively used as a model to study the molecular and cellular basis of nociception. The larval nociceptors, class IV dendritic arborization (C4da) neurons, line the body wall of the animal and respond to various stimuli including noxious heat and touch. Activation of C4da neurons results in a ...