Probe-Seq: Method for RNA Sequencing of Specific Cell Types from Animal Tissue
[Abstract] Most organs and tissues are composed of many types of cells. To characterize cellular state, various transcription profiling approaches are currently available, including whole-tissue bulk RNA sequencing, single cell RNA sequencing (scRNA-Seq), and cell type-specific RNA sequencing. What is missing in this repertoire is a simple, versatile method ...
Long-term Devocalization of Zebra Finches
Authors: Yunbok Kim,
Daisuke Mizuguchi and
Satoshi Kojima,
date: 09/20/2020,
view: 223,
Q&A: 0
[Abstract] Songbirds, such as the zebra finch, are a popular animal model for studying the neural basis of vocal and complex skill learning. Adult male zebra finches produce courtship song toward females (referred to as ‘directed song’) and recording and analyzing sounds of directed song along with underlying neural activity is important for investigating ...
Precise Targeting of Single Microelectrodes to Orientation Pinwheel Centers
[Abstract] In the mammalian visual system, early stages of visual form perception begin with orientation selective neurons in primary visual cortex (V1). In many species (including humans, monkeys, tree shrews, cats, and ferrets), these neurons are organized in pinwheel-like orientation columns. To study the functional organization within orientation ...
A Simple and Fast Battery Test for Phenotypic Characterization of Mice
Authors: Mariana S. A. Garcia-Gomes,
Dennis A. Zanatto,
Pedro K. Yamamoto,
Danilo Wadt,
Ana T. F. B. Antiorio,
Jilma Aleman-Laporte,
Sandra R. Alexandre-Ribeiro,
Guilherme A. Marson,
Cezar Guizzo,
Silvia M. G. Massironi,
Maria M. Bernardi and
Claudia M. C. Mori,
date: 04/05/2020,
view: 952,
Q&A: 0
[Abstract] Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or ...
Semi-automated Model to Accurately Counting Sympathetic Nervous Fibers
[Abstract] In recent years, the role of sympathetic nervous fibers in chronic inflammation has become increasingly evident. At the onset of inflammation, sympathetic activity is increased in the affected tissue. However, sympathetic fibers are largely absent from chronically inflamed tissues. Apparently, there is a very dynamic relationship between ...