Neuroscience

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    Protocols in Current Issue
    Minimally Invasive Oral Surgery Induction of the FRICT-ION Chronic Neuropathic Pain Model
    Authors:  Marena A. Montera and Karin N. Westlund, date: 04/20/2020, view: 352, Q&A: 0
    [Abstract] An easily induced preclinical trigeminal neuropathic nerve injury model is described here for the study of chronic pain, the model acronym FRICT-ION (Foramen Rotundum Inflammatory Constriction Trigeminal InfraOrbital Nerve). In patients, neuropathic pain is thought to be related to ...
    A Simple and Fast Battery Test for Phenotypic Characterization of Mice
    [Abstract] Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or ...
    Phototactic T-maze Behavioral Assay for Comparing the Functionality of Color-sensitive Photoreceptor Subtypes in the Drosophila Visual System
    Authors:  Hunter S. Shaw, Joe Larkin and Yong Rao, date: 03/20/2020, view: 273, Q&A: 0
    [Abstract] The Drosophila retina contains light-sensitive photoreceptors (R cells) with distinct spectral sensitivities that allow them to distinguish light by its spectral composition. R7 and R8 photoreceptors are important for color vision, and can be further classified into pale (p) or yellow (y) subtypes depending on the rhodopsin expressed. ...
    Retina Injury and Retina Tissue Preparation to Study Regeneration in Zebrafish
    Authors:  Poonam Sharma and Rajesh Ramachandran, date: 12/20/2019, view: 952, Q&A: 0
    [Abstract] Unlike mammals, primitive vertebrates have immense capability to regenerate almost all of their organs including the central nervous system. Among primitive organisms, zebrafish have been extensively used as a model system for regeneration studies. The retina is a part of the central nervous system and mammals lack the potential to repair any ...
    Semi-automated Model to Accurately Counting Sympathetic Nervous Fibers
    [Abstract] In recent years, the role of sympathetic nervous fibers in chronic inflammation has become increasingly evident. At the onset of inflammation, sympathetic activity is increased in the affected tissue. However, sympathetic fibers are largely absent from chronically inflamed tissues. Apparently, there is a very dynamic relationship between ...
    Evoked Potential Recordings of Auditory Brainstem Activity in the Mouse: An Optimized Method for the Assessment of Hearing Function of Mice
    Author:  Neil J. Ingham, date: 12/05/2019, view: 1028, Q&A: 0
    [Abstract] Hearing loss is a common sensory deficiency suffered by millions worldwide. It is a heterogeneous condition and genetics plays a critical role in its etiology. Gene variants can fundamentally alter hearing function, or predispose the auditory system towards loss of function resulting from other factors. In mouse studies of hearing loss and gene ...
    Optogenetic Food Odor Avoidance Assay
    Authors:  Jay M. Patel, Jessica Swanson and Benjamin R. Arenkiel, date: 10/20/2019, view: 829, Q&A: 0
    [Abstract] Appetite is tightly linked to the sensory experience of feeding, including the smell, taste, and sight of food. Sensory perception can affect the palatability of food, modulating appetite beyond homeostatic requirements. Hypothalamic neurons that govern feeding are responsive to sensory cues associated with food, including food odors. However, the ...
    Sciatic Nerve Cut and Repair Using Fibrin Glue in Adult Mice
    Authors:  Erica T. Akhter, Travis M. Rotterman, Arthur W. English and Francisco J. Alvarez, date: 09/20/2019, view: 1018, Q&A: 0
    [Abstract] Peripheral nerve injury (PNI) is an excellent model for studying neural responses to injury and elucidating the mechanisms that can facilitate axon regeneration. As such, several animal models have been employed to study regenerative mechanisms after PNI, including Aplysia, zebrafish, rabbits, cats and rodents. This protocol describes how to ...
    Cylinder Test to Assess Sensory-motor Function in a Mouse Model of Parkinson’s Disease
    [Abstract] Parkinson’s disease is a progressive neurodegenerative movement disorder that happens due to the loss of dopaminergic neurons in the substantia nigra. The deficiency of dopamine in the basal nuclei drives cardinal motor symptoms such as bradykinesia and hypokinesia. The current protocol describes the cylinder test, which is a relatively simple ...
    Whisker Nuisance Test: A Valuable Tool to Assess Tactile Hypersensitivity in Mice
    Authors:  Luigi Balasco, Gabriele Chelini, Yuri Bozzi and Giovanni Provenzano, date: 08/20/2019, view: 1249, Q&A: 0
    [Abstract] Abnormal response to tactile stimulation, described as both hyper- and hypo-reactivity, is a common sensory impairment in multiple neuropsychiatric disorders. The neural bases of tactile sensitivity remain so far unknown. In the last years, animal studies have proven to be useful for shedding light on the cellular and molecular mechanism ...



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