Protocols in Current Issue
    Headpost Surgery for in vivo Electrophysiological Recording in the Mouse Inferior Colliculus during Locomotion
    Authors:  Yoonsun Yang and Gunsoo Kim, date: 12/05/2020, view: 378, Q&A: 0

    The inferior colliculus (IC) is a critical midbrain integration center for auditory and non-auditory information. Although much is known about the response properties of the IC neurons to auditory stimuli, how the IC neural circuits function during movement such as locomotion remains poorly understood. Mice offer a valuable model in this respect,

    Probe-Seq: Method for RNA Sequencing of Specific Cell Types from Animal Tissue
    [Abstract] Most organs and tissues are composed of many types of cells. To characterize cellular state, various transcription profiling approaches are currently available, including whole-tissue bulk RNA sequencing, single cell RNA sequencing (scRNA-Seq), and cell type-specific RNA sequencing. What is missing in this repertoire is a simple, versatile method ...
    Long-term Devocalization of Zebra Finches
    Authors:  Yunbok Kim, Daisuke Mizuguchi and Satoshi Kojima, date: 09/20/2020, view: 1276, Q&A: 0
    [Abstract] Songbirds, such as the zebra finch, are a popular animal model for studying the neural basis of vocal and complex skill learning. Adult male zebra finches produce courtship song toward females (referred to as ‘directed song’) and recording and analyzing sounds of directed song along with underlying neural activity is important for investigating ...
    Sequential Reaching Task for the Study of Motor Skills in Monkeys
    Authors:  Machiko Ohbayashi and Nathalie Picard, date: 08/20/2020, view: 764, Q&A: 0
    [Abstract] The ability to perform a sequence of movements is a key component of motor skills, such as typing or playing a musical instrument. How the brain binds elementary movements together into meaningful actions has been a topic of much interest. Here, we describe two sequential reaching tasks that we use to investigate the neural substrate of skilled ...
    A Reproducible Protocol to Measure the Critical Swimming Speed of Adult Zebrafish
    Authors:  Yuma Wakamatsu, Makoto Kashima and Hiromi Hirata, date: 08/20/2020, view: 1030, Q&A: 0
    [Abstract] The quantitative measurement of water flow-induced swimming of fish species using a swimmill is a powerful method to evaluate motor ability of individual fish. Zebrafish is a commonly used vertebrate that enables the study of morphological, physiological and behavioral characteristics associated with genes. We here established a reproducible ...
    Precise Targeting of Single Microelectrodes to Orientation Pinwheel Centers
    Authors:  Xue Mei Song, Ming Li, Tao Xu, Dewen Hu and Anna Wang Roe, date: 06/05/2020, view: 793, Q&A: 0
    [Abstract] In the mammalian visual system, early stages of visual form perception begin with orientation selective neurons in primary visual cortex (V1). In many species (including humans, monkeys, tree shrews, cats, and ferrets), these neurons are organized in pinwheel-like orientation columns. To study the functional organization within orientation ...
    Minimally Invasive Oral Surgery Induction of the FRICT-ION Chronic Neuropathic Pain Model
    Authors:  Marena A. Montera and Karin N. Westlund, date: 04/20/2020, view: 1259, Q&A: 0
    [Abstract] An easily induced preclinical trigeminal neuropathic nerve injury model is described here for the study of chronic pain, the model acronym FRICT-ION (Foramen Rotundum Inflammatory Constriction Trigeminal InfraOrbital Nerve). In patients, neuropathic pain is thought to be related to ...
    A Simple and Fast Battery Test for Phenotypic Characterization of Mice
    [Abstract] Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or ...
    Phototactic T-maze Behavioral Assay for Comparing the Functionality of Color-sensitive Photoreceptor Subtypes in the Drosophila Visual System
    Authors:  Hunter S. Shaw, Joe Larkin and Yong Rao, date: 03/20/2020, view: 1252, Q&A: 0
    [Abstract] The Drosophila retina contains light-sensitive photoreceptors (R cells) with distinct spectral sensitivities that allow them to distinguish light by its spectral composition. R7 and R8 photoreceptors are important for color vision, and can be further classified into pale (p) or yellow (y) subtypes depending on the rhodopsin expressed. ...
    Retina Injury and Retina Tissue Preparation to Study Regeneration in Zebrafish
    Authors:  Poonam Sharma and Rajesh Ramachandran, date: 12/20/2019, view: 1948, Q&A: 0
    [Abstract] Unlike mammals, primitive vertebrates have immense capability to regenerate almost all of their organs including the central nervous system. Among primitive organisms, zebrafish have been extensively used as a model system for regeneration studies. The retina is a part of the central nervous system and mammals lack the potential to repair any ...

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