Laser Capture Micro-dissection (LCM) of Neonatal Mouse Forebrain for RNA Isolation
[Abstract] Precise and reproducible isolation of desired cell types or layers from heterogeneous tissues is crucial to analyze specific gene profiles and molecular interactions in vivo. Forebrain is the core site of higher functions, like cognition and memory consolidation. It is composed of heterogeneous and distinct cell types, interconnected to ...
Gallyas Silver Impregnation of Myelinated Nerve Fibers
[Abstract] In the nervous system of vertebrates, nerve impulse propagation is accelerated by the ensheathment of neuronal axons with myelin. Myelin sheaths are molecularly specialized, lipid-rich plasma membrane extensions of Schwann cells in the peripheral nervous system and oligodendrocytes in the central nervous system (CNS). To visualize myelinated nerve ...
Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation
Authors: Mariko Kinoshita-Kawada,
Hiroshi Hasegawa,
Tsunaki Hongu,
Shigeru Yanagi,
Yasunori Kanaho,
Ichiro Masai,
Takayasu Mishima,
Xiaoping Chen,
Yoshio Tsuboi,
Yi Rao,
Junichi Yuasa-Kawada and
Jane Y. Wu,
date: 09/20/2019,
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[Abstract] Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response. Although primary neuron culture has been a versatile technique for elucidating various developmental mechanisms, many in ...
A Modified Barnes Maze for Juvenile Rats
[Abstract] To better understand neural codes for spatial navigation and to address cognitive development questions, the neurobiology of postnatal hippocampal development is receiving increased attention. While the Morris Water Maze is the gold standard for assessing hippocampal integrity, potential confounds can be difficult to control for in juvenile ...
In utero Electroporation of Mouse Cerebellar Purkinje Cells
Author: Yukari H. Takeo,
date: 06/05/2016,
view: 10332,
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[Abstract] In utero electroporation (IUE) of mouse cerebellar Purkinje cells allows high expression levels of transgenes without toxicity (Nishiyama et al., 2012). This technique is suitable for co-transfection of multiple plasmid genes. Therefore, it is useful to express various sets of genes such as drug-inducible Cre/loxP constructs and ...