Search

Cell Biology

Categories

    Protocols in Current Issue
    Micro-computed Tomography to Visualize Vascular Networks in Maize Stems
    Authors:  Akiteru Maeno and Katsutoshi Tsuda, date: 01/05/2018, view: 174, Q&A: 0
    [Abstract] Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular systems, especially in crop species, have been poorly described since they are deeply embedded in the ...
    A Rodent Model for Chronic Brain Hypoperfusion Related Diseases: Permanent Bilateral Occlusion of the Common Carotid Arteries (2VO) in Rats
    Authors:  Mei-Ling Yan and Jing Ai, date: 01/05/2018, view: 120, Q&A: 0
    [Abstract] Permanent occlusion of bilateral common carotid arteries (2VO) in rat is considered as a suitable animal model to mimic chronic brain hypoperfusion status, which is proved to be a risk factor to precede the Alzheimer’s disease or/and vascular dementia. In this protocol, we describe how to successfully ligate the bilateral common carotid arteries ...
    Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery
    Authors:  Damayanti Chakraborty, Masanaga Muto and Michael J. Soares, date: 12/20/2017, view: 256, Q&A: 0
    [Abstract] In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of ‘gain-of-function’ and ‘loss-of-function’ blastocyst ...
    Mouse Model of Immune Complex-mediated Vasculitis in Dorsal Skin and Assessment of the Neutrophil-mediated Tissue Damage
    [Abstract] Neutrophils are the most abundant leukocytes in the blood. In the recent decades, their crucial roles in host defense, immune regulation and tissue damage have been studied in a deeper dimension. In this protocol, we described a mouse model of immune complex-mediated vasculitis in the dorsal skin induced by Arthus reaction, and the subsequent ...
    Investigating the Shape of the Shoot Apical Meristem in Bamboo Using a Superellipse Equation
    Authors:  Qiang Wei and Peijian Shi, date: 12/05/2017, view: 285, Q&A: 0
    [Abstract] The shoot apical meristem is the origin of bamboo wood. Its structure and morphology are important for maintaining the normal development of bamboo wood. However, the traditional method to describe the morphology of the shoot apical meristem in bamboo or other plants only depends on qualitative approaches. Here we present a protocol for precisely ...
    zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain
    [Abstract] In studies of brain function, it is essential to understand the underlying neuro-architecture. Very young zebrafish larvae are widely used for neuroarchitecture studies, due to their size and natural transparency. However, this model system has several limitations, due to the immaturity, high rates of development and limited behavioral repertoire ...
    Mandibular Explant Assay for Investigating Extrinsic Stimuli on Bone and Cartilage Development
    Authors:  Jamie Jiang, Jessica W. Bertol and Walid D. Fakhouri, date: 12/05/2017, view: 367, Q&A: 0
    [Abstract] A major issue in developmental biology is to determine how embryonic tissues respond to molecular signals in a timely manner and given the position-restricted instructions during morphogenesis, of which Meckel’s cartilage is a classical example. The ex-vivo explant model is a practical and convenient system that allows investigation of ...
    Automatic Leaf Epidermis Assessment Using Fourier Descriptors in Texture Images
    [Abstract] The identification of plant species is not a trivial task, since it is carried out by experts and depends on the presence of fruits, flowers and leaves. However, fruits and flowers are not available throughout the year, while leaves are accessible most of the year. In order to assist the specialized work of species identification, methods of ...
    [Bio101] RNA Extraction from Wistar Rat Cochlea for qRT-PCR
    Authors:  Janaína Cândida Rodrigues and Rubens Vuono de Brito Neto, date: 12/05/2017, view: 243, Q&A: 0
    [Abstract] Otology research has developed considerably in recent years and molecular analysis is crucial to identify metabolic pathways and therapeutic targets. However, the structure of the cochlea limits the amount of cell mass, and special care is required for RNA extraction. Studies applying this technique to the cochlea are scarce in the literature, and ...
    Instillation of Particulate Suspensions to the Lungs
    Authors:  Etsushi Kuroda, Yasuo Morimoto and Ken J Ishii, date: 11/20/2017, view: 626, Q&A: 0
    [Abstract] Inhaled fine particulates are thought to cause chronic pulmonary inflammation through the deposition of particulates into the lungs. To investigate the effect of fine particulates on the lungs, instillation of suspension of particulates into the lungs is required. This protocol describes direct injection of suspensions of fine particulates into ...