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Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

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In vitro Differentiation of Thymic T_reg Cell Progenitors to Mature Thymic T_reg Cells

Authors: David L. Owen and Michael A. Farrar, date: 08/20/2019, view: 1871, Q&A: 0

[Abstract] Thymic T_reg cell differentiation occurs via a two-step process. Step one generates T_reg cell progenitors (T_regP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated by activation of STAT5 via IL-2, or IL-15, leading to mature T_reg cells capable of ...

Induced Germinal Center B Cell Culture System

Authors: Kei Haniuda and Daisuke Kitamura, date: 02/20/2019, view: 7523, Q&A: 0

[Abstract] The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly because experimental manipulation of GC B cells in vivo ...

Ultrasound Guided Intra-thymic Injection to Track Recent Thymic Emigrants and Investigate T Cell Development

Authors: Haiguang Wang, David L. Owen, Lily J. Qian, Laura B. Chopp, Michael A. Farrar and Kristin A. Hogquist, date: 12/05/2018, view: 2553, Q&A: 0

[Abstract] To track recent thymic emigrants (RTEs) or study T cell development in the thymus, intra-thymic injection of a cellular tag or precursor cells for various T cell lineages is often desired. However, the traditional surgical approach to expose the thymus for intra-thymic injection is time-consuming and can cause a high level of pain and stress to ...

High Dimensional Functionomic Analysis of Human Hematopoietic Stem and Progenitor Cells at a Single Cell Level

Authors: Thomas Luh, Kimberly Lucero, Wenji Ma, Jaeyop Lee, Yu Jerry Zhou, Yufeng Shen and Kang Liu, date: 05/20/2018, view: 4641, Q&A: 0

[Abstract] The ability to conduct investigation of cellular transcription, signaling, and function at the single-cell level has opened opportunities to examine heterogeneous populations at unprecedented resolutions. Although methods have been developed to evaluate high-dimensional transcriptomic and proteomic data (relating to cellular mRNA and protein), ...

Generation of Busulfan Chimeric Mice for the Analysis of T Cell Population Dynamics

Authors: Thea Hogan, Andrew Yates and Benedict Seddon, date: 12/20/2017, view: 6940, Q&A: 0

[Abstract] This protocol was developed to generate chimeric mice in which T lymphocytes could be stratified by age on the basis of congenic marker expression. The conditioning drug busulfan is used to ablate host haematopoietic stem cells while leaving the peripheral immune system intact. Busulfan treatment is followed by bone marrow transplantation (BMT), ...

In vitro Homeostatic Proliferation of Human CD8 T Cells

Authors: Hossam A. Abdelsamed, Caitlin C. Zebley and Ben Youngblood, date: 11/20/2017, view: 7463, Q&A: 0

[Abstract] Long-lived T-cell–mediated immunity requires persistence of memory T cells in an antigen-free environment while also maintaining a heightened capacity to recall effector functions. Such antigen-independent homeostatic proliferation is mediated in part by the common gamma-chain cytokines IL-7 and IL-15. To further explore the mechanisms governing ...

Differentiation of Myeloid-derived Suppressor Cells from Murine Bone Marrow and Their Co-culture with Splenic Dendritic Cells

Authors: Giada Mondanelli and Claudia Volpi, date: 09/20/2017, view: 8039, Q&A: 0

[Abstract] Myeloid-derived suppressor cells (MDSCs) possess the ability to suppress the immune response, and to amplify the regulatory properties of other immune cells, i.e., dendritic cells. Here we describe a protocol in which MDSCs were differentiated from murine bone marrow cells, and CD11c⁺ dendritic cells were purified from murine ...

Efficient Production of Functional Human NKT Cells from Induced Pluripotent Stem Cells − Reprogramming of Human Vα24⁺iNKT Cells

Authors: Daisuke Yamada, Tomonori Iyoda, Kanako Shimizu, Yusuke Sato, Haruhiko Koseki and Shin-ichiro Fujii, date: 05/20/2017, view: 8534, Q&A: 0

[Abstract] Antigen-specific T cell-derived induced pluripotent stem cells (iPSCs) have been shown to re-differentiate into functional T cells and thus provide a potential source of T cells that could be useful for cancer immunotherapy. Human Vα24⁺ invariant natural killer T (Vα24⁺iNKT) cells are subset of T cells that are characterized ...

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