Calvarial Bone Implantation and in vivo Imaging of Tumor Cells in Mice
[Abstract] Bone is one of common metastasis sites for many types of cancer. In bone metastatic microenvironment, tumor-bone interactions play a significant role in the regulation of osteolytic or osteoblastic bone metastasis. In order to investigate the direct interaction between tumor cells and bone tissue, it is essential to generate appropriate animal ...
Zebrafish Embryo Xenograft and Metastasis Assay
[Abstract] Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. ...
Intra-amniotic Injection of Mouse Embryos
[Abstract] Recent outbreaks of infectious neuro-developmental diseases such as congenital Zika syndrome - have led to a demand for prognosis data from animal models. We developed an intra-amniotic injection mice model that allows Zika virus (ZIKV) infected mice to grow to puberty. In this system, ZIKV is injected into the amniotic fluid of pregnant mice and ...
Murine Pancreatic Islets Transplantation under the Kidney Capsule
[Abstract] Type 1 diabetes (T1D) is an autoimmune disease caused by the lack of insulin-producing pancreatic beta cells leading to systemic hyperglycemia. Pancreatic islet transplantation is a valid therapeutic approach to restore insulin loss and to promote adequate glycemic control. Pancreatic islet transplantation in mice is an optimal preclinical model ...
Mouse Satellite Cell Isolation and Transplantation
Authors: Elia Angelino,
Simone Reano,
Michele Ferrara,
Emanuela Agosti,
Hana Sustova,
Valeria Malacarne,
Sara Clerici,
Andrea Graziani and
Nicoletta Filigheddu,
date: 01/20/2018,
view: 4521,
Q&A: 0
[Abstract] Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ...
Xenograft Mouse Model of Human Uveal Melanoma
Authors: Yao Chen,
Xiao Liu,
Ling Gao and
Yongqing Liu,
date: 11/05/2017,
view: 3850,
Q&A: 0
[Abstract] Uveal melanoma (UM) is a malignant intraocular tumor in adults. Metastasis develops in almost half of the patients and over 90% of the metastases are in the liver. With the advances in molecular targeting therapy for melanoma, a proper metastasis animal model is of increasing importance for testing the accuracy and effectiveness of systemic ...
Improved Oviduct Transfer Surgery for Genetically Modified Rat Production
[Abstract] Rat embryo transfer surgeries are becoming more common with targeted nucleases increasing the demand for rat models. This protocol details pre-surgical preparation, improved surgical techniques for placing embryos into the oviduct, and post-surgical care of rats to parturition. Direct application of mouse oviduct transfer protocols results in ...
Tumorigenicity Assay in Nude Mice
Authors: Feng Du,
Xiaodi Zhao and
Daiming Fan,
date: 07/05/2017,
view: 11460,
Q&A: 0
[Abstract] Tumorigenicity refers to the ability of cultured cells to develop viable tumors in immune-deficient animals. The goal of this protocol is to illustrate tumorigenicity assay by subcutaneous tumor-cell-transplantation in nude mice. Target cells are transplanted to 6-week-old nude mice subcutaneously and the tumor growth is monitored over a period of ...
Intracaecal Orthotopic Colorectal Cancer Xenograft Mouse Model
Authors: Hsin-Wei Liao and
Mien-Chie Hung,
date: 06/05/2017,
view: 6842,
Q&A: 1
[Abstract] The host microenvironment plays a prominent role in tumor growth, angiogenesis, invasion, metastasis, and response to therapy. Orthotopic tumor model mimics the natural environment of tumor development and provides an effective approach to investigate tumor pathophysiology and develop therapeutic strategies. This protocol describes the technique ...